The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells
The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells
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DOI：
10.1016/s0092-8674(00)80240-8
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发表时间：
1997-05-16
期刊：
影响因子：
64.5
通讯作者：
Flavell, RA
中科院分区：
文献类型：
--
作者：
Zheng, WP;Flavell, RA
CD4T细胞分别通过Th1和Th2细胞的作用增强炎症或体液免疫反应。然而，这些细胞从幼稚T细胞前体分化的分子基础尚不清楚。我们发现GATA-3在Th2细胞中选择性表达。GATA-3在幼稚、新激活的T细胞和Th2系细胞中高水平表达，但随着幼稚细胞致力于其Th亚群，在Th1系细胞中表达降至最低水平。反义GATA-3抑制Th2克隆D10中所有Th2细胞因子基因的表达。GATA-3在M12细胞中直接激活IL-4启动子-荧光素酶报告基因。在转基因小鼠中，CD4T细胞中GATA-3的升高导致发育中的Th1细胞中Th2细胞因子基因的表达。因此，GATA-3是Th2细胞因子基因表达的必要条件和充分条件。
CD4 T cells potentiate the inflammatory or humoral immune response through the action of Th1 and Th2 cells, respectively. The molecular basis of the differentiation of these cells from naive T cell precursors is, however, unclear. We found that GATA-3 was selectively expressed in Th2 cells. GATA-3 is expressed at a high level in naive, freshly activated T cells and Th2 lineage cells, but subsides to a minimal level in Th1 lineage cells as naive cells commit to their Th subset. Antisense GATA-3 inhibited the expression of all Th2 cytokine genes in the Th2 clone D10. GATA-3 directly activated an IL-4 promoter-luciferase reporter gene in M12 cells. In transgenic mice, elevated GATA-3 in CD4 T cells caused Th2 cytokine gene expression in developing Th1 cells. Thus, GATA-3 is necessary and sufficient for Th2 cytokine gene expression.