Raman Spectroscopy Methods to Characterize the Mechanical Response of Soft Biomaterials
Raman Spectroscopy Methods to Characterize the Mechanical Response of Soft Biomaterials
复制标题
表征软生物材料机械响应的拉曼光谱方法
DOI：
10.1021/acs.biomac.0c00818
复制
发表时间：
2020
影响因子：
6.2
通讯作者：
Subhash, Ghatu
中科院分区：
文献类型：
--
作者：
Zhou, Hui;Simmons, Chelsey S.;Sarntinoranont, Malisa;Subhash, Ghatu
拉曼光谱已被广泛用于表征机械变形对生物材料微观结构变化的影响。虽然传统的压电谱学已经成功地通过跟踪显著的峰移来评估硬生物材料的内应力，但对于模数在千到兆帕斯卡范围内的软生物材料，由于外加载荷引起的峰移接近或低于光谱仪的分辨率极限。在这篇综述中，除了峰位移，还讨论了其他光谱特征(例如，极化强度和强度比)，这些特征提供了对软生物材料的微结构取向和二级结构及其应变相关性的定量评估。我们为每种方法提供了具体的例子，并对自然(例如软组织)和合成(例如聚合物支架)软生物材料在机械变形时常见的敏感拉曼特征带进行了分类。这一综述可以为利用拉曼光谱分析软组织和工程化组织结构的微观力学的研究人员提供指导。
Raman spectroscopy has been used extensively to characterize the influence of mechanical deformation on microstructure changes in biomaterials. While traditional piezo-spectroscopy has been successful in assessing internal stresses of hard biomaterials by tracking prominent peak shifts, peak shifts due to applied loads are near or below the resolution limit of the spectrometer for soft biomaterials with moduli in the kilo- to mega-Pascal range. In this Review, in addition to peak shifts, other spectral features (e.g., polarized intensity and intensity ratio) that provide quantitative assessments of microstructural orientation and secondary structure in soft biomaterials and their strain dependence are discussed. We provide specific examples for each method and classify sensitive Raman characteristic bands common across natural (e.g., soft tissue) and synthetic (e.g., polymeric scaffolds) soft biomaterials upon mechanical deformation. This Review can provide guidance for researchers aiming to analyze micromechanics of soft tissues and engineered tissue constructs by Raman spectroscopy.