Posterolateral intertransverse lumbar fusion in a mouse model: surgical anatomy and operative technique
Posterolateral intertransverse lumbar fusion in a mouse model: surgical anatomy and operative technique
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DOI：
10.1016/j.spinee.2006.03.004
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发表时间：
2007-01-01
期刊：
影响因子：
4.5
通讯作者：
Cooley, Brian C.
中科院分区：
文献类型：
--
作者：
Rao, Raj D.;Bagaria, Vaibhav B.;Cooley, Brian C.
背景：动物模型经常用于研究骨移植替代物或同种异体材料对骨外侧腰椎融合的影响。转基因技术在小鼠提供了一个独特的机会，进一步了解脊柱融合的生物学。目的：描述相关的腰椎解剖并制定小鼠后外侧融合模型的手术方案。研究设计：诊断模型：建立用于后外侧脊柱融合生物学评估的动物模型。方法：处死10只小鼠，研究相关腰椎解剖，制定腰椎融合方案。在46只小鼠中验证了L4-L6融合方案，以方便暴露、准备后外侧融合床、引入骨诱导剂和围手术期护理。结果：描述了小鼠后外侧腰椎横间融合模型的解剖和手术技术。椎旁入路可以暴露横突，去皮，并在L4-L6横间融合部位放置移植物。单纯去皮不能产生融合，而骨移植物的使用可以产生令人满意的融合率。围手术期发病率和死亡率均较低。结论：小鼠后外侧腰椎融合模型重复性好，成本低，并发症发生率低。对相关解剖的了解和对明确的手术方案的遵守提供了一个可靠和可重复的脊柱融合实验模型。(C) 2007爱思唯尔公司版权所有。
BACKGROUND CONTEXT: Animal models are frequently used for studying the effect of bone graft substitutes or allogeneic materials on osterolateral lumbar fusion. Transgenic technology in the mouse provides a unique opportunity to further understand the biology of spine fusion.PURPOSE: To describe pertinent lumbar spine anatomy and formulate a surgical protocol for posterolateral fusion in the mouse model.STUDY DESIGN: Diagnostic model: development of an animal model for biologic evaluation of posterolateral spine fusion.METHOD: Ten mice were killed to study relevant lumbar spine anatomy and develop a protocol for lumbar spine fusion. The L4-L6 fusion protocol was validated in 46 mice for ease of exposure, preparation of the posterolateral fusion bed, introduction of bone inductive agents, and perioperative care.RESULTS: Anatomy and surgical technique for posterolateral intertransverse lumbar fusion in the mouse model are described. A paraspinal approach allows exposure of the transverse processes, decortication, and graft placement at the L4-L6 intertransverse fusion site. Decortication alone did not result in fusion, whereas the use of bone graft resulted in satisfactory fusion rates. Perioperative morbidity and mortality rates were low.CONCLUSION: The mouse posterolateral lumbar spine fusion model is reproducible, inexpensive, and has low complication rates. Knowledge of the relevant anatomy and adherence to a well-defined surgical protocol provides a reliable and reproducible experimental spine fusion model. (C) 2007 Elsevier Inc. All rights reserved.