Avian leukosis virus subgroup A and B infection in wild birds of Northeast China
Avian leukosis virus subgroup A and B infection in wild birds of Northeast China
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DOI：
10.1016/j.vetmic.2013.01.020
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发表时间：
2013-05-03
影响因子：
3.3
通讯作者：
Gao, Yulong
中科院分区：
文献类型：
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作者：
Li, Delong;Qin, Liting;Gao, Yulong
为了分析中国野禽感染禽流感白血病病毒（ALV）的情况，我们采集了300只来自不同地区的野禽。病毒分离和PCR结果表明，野生鸟类感染了ALV-A和ALV-B病毒。分别用ALV-A和-B引物进行PCR检测，得到2个ALV-A和4个ALV-B环境序列。结果表明，2株ALV-A株的gp85基因与RAV-1的同源性最高，达到99.8%，与其他美洲株的同源性超过92%。而两株ALV-A的gp85基因与中国株的同源性略低（87.2-92.6%）。4株ALV-B与原型株（rav2）同源性较高，为99.1% ~ 99.4%，与Schmidt-Ruppin B和Prague亚群同源性较低，为93.3% ~ 98.4%。4株ALV-B与SDAU09C2和SDAU09E3的同源性最低（90%）。总之，这些结果表明禽流感病毒已经感染了中国的野生鸟类。(C) 2013 Elsevier B.V.版权所有
To analyze the status of wild birds infected with avian leukosis virus (ALV) in China, we collected 300 wild birds from various areas. Virus isolation and PCR showed that wild birds were infected by ALV-A and ALV-B. Two ALV-A and 4 ALV-B env sequences were obtained by PCR using primers designed to detect ALV-A and -B respectively. Our results showed that the gp85 genes of the 2 ALV-A strains have the highest homology with RAV-1, 99.8%, and more than 92% homology with other American strains. However, the gp85 genes of the two ALV-A strains showed slightly lower homology with Chinese strains (87.2-92.6%). Additionally, the 4 ALV-B strains have high homology with the prototype strain (RAV-2), from 99.1 to 99.4%, but they have slightly lower identity with Schmidt-Ruppin B and Prague subgroup B, from 93.3 to 98.4%. The 4 ALV-B strains showed the lowest identity with SDAU09C2 and SDAU09E3 (90%). In total, these results suggested that avian leukosis virus has infected wild birds in China. (C) 2013 Elsevier B.V. All rights reserved.