LAMININ, TYPE-IV COLLAGEN, AND FIBRONECTIN IN NORMAL AND CRYPTORCHID HUMAN TESTES - AN IMMUNOHISTOCHEMICAL STUDY
LAMININ, TYPE-IV COLLAGEN, AND FIBRONECTIN IN NORMAL AND CRYPTORCHID HUMAN TESTES - AN IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1111/j.1365-2605.1990.tb00970.x
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发表时间：
1990-04-01
影响因子：
--
通讯作者：
NISTAL, M
中科院分区：
文献类型：
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作者：
SANTAMARIA, L;MARTINEZONSURBE, P;NISTAL, M
层粘连蛋白，IV型胶原蛋白和纤维连接蛋白的免疫组织化学研究进行了正常男性睾丸和隐睾和对侧阴囊隐睾男性从2岁到40岁。在免疫染色切片中测量固有层每单位面积的积分光密度（IOD）。纤维连接蛋白在整个生精小管的固有层厚度和间质结缔组织中被发现。层粘连蛋白在生精小管固有层的最内层及毛细血管内皮周围均有表达。在青春期前，正常和隐睾睾丸之间没有差异。在成人隐睾，层粘连蛋白形成更多的和更深的内陷向生精上皮比正常成人睾丸。IV型胶原蛋白出现在正常睾丸固有层的整个厚度以及间质血管壁中。从婴儿期开始，生精小管的固有层（而不是血管壁）显示IV型胶原蛋白的免疫染色较少，并且该组分的IOD较同龄男性的对照试验低。隐睾对侧阴囊睾丸IV型胶原免疫组化染色介于正常对照组和隐睾组之间。这些发现表明隐睾患者和对侧阴囊睾丸的生精小管固有层在早期就已受损。
An immunohistochemical study of laminin, type IV collagen, and fibronectin was carried out in the testes of normal men and in the cryptorchid and contralateral scrotal testes of cryptorchid men from 2 to 40 years of age. The integrated optical density (IOD) per unit area of the lamina propria was measured in the immunostained sections. Fibronectin was found throughout the thickness of the lamina propria of the seminiferous tubules and in the interstitial connective tissue. No differences between normal and cryptorchid testes were found. Laminin was observed in the innermost part of the lamina propria of the seminiferous tubules and surrounding the endothelium of blood capillaries from infancy. No differences were found between normal and cryptorchid testes in the prepubertal period. In adult cryptorchid testes, laminin formed more numerous and deeper invaginations towards the seminiferous epithelium than in normal adult testes. Type IV collagen appeared throughout the thickness of the lamina propria of normal testes as well as in the wall of interstitial blood vessels. From infancy, the lamina propria of seminiferous tubules, but not blood vessel walls, showed lesser immunostaining for type IV collagen and a lower IOD of this component than did control tests from men of the same age. No differences between unilateral and bilateral cryptorchidism were found. The contralateral scrotal testes of cryptorchid males showed intermediate immunostaining for type IV collagen between that of normal control testes and that of cryptorchid testes. These findings suggest that the lamina propria of seminiferous tubules is lesioned at an early age in both cryptorchid and contralateral scrotal testes of cryptorchid men.