Morphological and biochemical changes of lymphatic vessels in the soleus muscle of mice after hindlimb unloading
Morphological and biochemical changes of lymphatic vessels in the soleus muscle of mice after hindlimb unloading
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DOI：
10.1002/mus.27402
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发表时间：
2021-08-28
期刊：
影响因子：
3.4
通讯作者：
Kawakami，Keisuke
中科院分区：
文献类型：
--
作者：
Kawashima，Takafumi;Ji，Rui-Cheng;Kawakami，Keisuke
方法：雄性C57 BL/6小鼠尾部悬吊2周或4周，诱导比目鱼肌萎缩。TS后，比目鱼肌中的淋巴管和毛细血管通过LYVE-1和CD 31的双重染色进行可视化和计数。分别采用Western blotting和实时逆转录聚合酶链反应检测血管内皮生长因子（VEGF）-C、VEGF-D和血管内皮生长因子受体-3的蛋白和mRNA水平。
Methods: Male C57BL/6 mice were subjected to tail suspension (TS) for 2 or 4 weeks to induce soleus muscle atrophy. After TS, lymphatic and blood capillaries in the soleus muscle were visualized and counted by double staining with LYVE-1 and CD31. The protein and mRNA levels of vascular endothelial growth factor (VEGF)-C, VEGF-D, and vascular endothelial growth factor receptor-3 were measured by Western blotting and real-time reverse transcript polymerase chain reaction, respectively.