Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan
Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan
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DOI：
10.1016/j.ttbdis.2017.03.007
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发表时间：
2017-01-01
影响因子：
3.2
通讯作者：
Matsuo, Tomohide
中科院分区：
文献类型：
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作者：
Masatani, Tatsunori;Hayashi, Kei;Matsuo, Tomohide
为了揭示蜱传寄生虫的分布，我们建立了一种新的巢式聚合酶链反应（PCR）系统来检测蜱传寄生虫病的最常见的病原体，即巴伯虫，泰勒虫和肝虫寄生虫。我们在日本西南部九州岛最南端的鹿儿岛县收集了寻找宿主或取食动物的蜱。在776份蜱类样品中，有20份（20/776：2.58%）在巢式PCR中显示了18 SrRNA基因的适当大小（约1.4-1.6 kbp）的特异性条带。这些PCR产物具有巴氏杆菌属的个体序列。(from 8蜱）、泰勒虫属（Theileria spp.）(from 9只蜱：1份蜱样本包括至少2种泰勒虫属。序列）和肝虫属（Hepatozoon spp.）(from 3个滴答声）。系统发育分析表明，这些序列接近那些未描述的Babalypspp。在日本的野生浣熊中检测到的（5个序列; 3个序列相同），在中国的猪中检测到的吉氏巴氏杆菌样寄生虫（3个序列;所有序列相同），泰勒虫属（Theileria spp.）在日本和中国的西卡鹿（10个序列; 2个序列相同）、肝体动物（1个序列）和肝体动物属中检出。在日本的日本貂中检测到（两个序列）。总之，我们表明，各种蜱传寄生虫存在于鹿儿岛，在日本南部地区，通过使用新的巢式PCR系统。这些包括未描述的物种，如先前在中国猪中检测到的吉氏巴氏杆菌样寄生虫。重要的是，我们的研究结果揭示了日本南部蜱虫和原生动物寄生虫的新组合。本研究的结果将有助于识别由蜱传寄生虫引起的潜在寄生虫性动物疾病。
To reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (PCR) system to detect the most common agents of tick-borne parasitic diseases, namely Babesia, Theileria, and Hepatozoon parasites. We collected host-seeking or animal-feeding ticks in Kagoshima Prefecture, the southern-most region of Kyusyu Island in southwestern Japan. Twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6 kbp) for the 18S rRNA genes in the novel nested PCR (20/776: 2.58%). These PCR products have individual sequences of Babesia spp. (from 8 ticks), Theileria spp. (from 9 ticks: one tick sample including at least two Theileria spp. sequences), and Hepatozoon spp. (from 3 ticks). Phylogenetic analyses revealed that these sequences were close to those of undescribed Babesia spp. detected in feral raccoons in Japan (5 sequences; 3 sequences being identical), Babesia gibsoni-like parasites detected in pigs in China (3 sequences; all sequences being identical), Theileria spp. detected in sika deer in Japan and China (10 sequences; 2 sequences being identical), Hepatozoon cans (one sequence), and Hepatozoon spp. detected in Japanese martens in Japan (two sequences). In summary, we showed that various tick-borne parasites exist in Kagoshima, the southern region in Japan by using the novel nested PCR system. These including undescribed species such as Babesia gibsoni-like parasites previously detected in pigs in China. Importantly, our results revealed new combinations of ticks and protozoan parasites in southern Japan. The results of this study will aid in the recognition of potential parasitic animal diseases caused by tick-borne parasites.