Membrane architecture and adherens junctions contribute to strong Notch pathway activation
Membrane architecture and adherens junctions contribute to strong Notch pathway activation
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膜结构和粘附连接有助于Notch通路的强烈激活
DOI：
10.1101/2021.05.26.445755
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Falo-Sanjuan J
中科院分区：
文献类型：
--
作者：
Falo-Sanjuan J
Notch通路介导多种组织、发育阶段和生物体中的细胞间通讯。通路激活依赖于跨膜配体和相邻细胞上的受体之间的相互作用。因此，通路活性可能受到膜之间接触的大小、组成或动力学的影响。Notch信号在theDrosophilaembryo的启动发生在细胞化过程中，当侧细胞膜和adherens连接第一次被沉积，使我们能够调查膜结构和特定的连接域的信号的重要性。通过测量Notch依赖的转录在活胚胎中，我们建立了它启动，而侧膜生长和信号的发病与其形成的特定阶段。然而，侧膜本身的长度没有限制。相反，与膜沉积同时组装的粘附连接有助于转录所需的高水平信号传导，如α-连环蛋白耗尽的结果所示。总之，这些结果表明，侧膜接触的建立可以限制Notch反式激活，并表明粘附连接在调节Notch活性中起重要作用。
The Notch pathway mediates cell-to-cell communication in a variety of tissues, developmental stages and organisms. Pathway activation relies on the interaction between transmembrane ligands and receptors on adjacent cells. As such, pathway activity could be influenced by the size, composition or dynamics of contacts between membranes. The initiation of Notch signalling in theDrosophilaembryo occurs during cellularization, when lateral cell membranes and adherens junctions are first being deposited, allowing us to investigate the importance of membrane architecture and specific junctional domains for signalling. By measuring Notch-dependent transcription in live embryos, we established that it initiates while lateral membranes are growing and that signalling onset correlates with a specific phase in their formation. However, the length of the lateral membranes per se was not limiting. Rather, the adherens junctions, which assemble concurrently with membrane deposition, contributed to the high levels of signalling required for transcription, as indicated by the consequences of α-Catenin depletion. Together, these results demonstrate that the establishment of lateral membrane contacts can be limiting for Notch trans-activation and suggest that adherens junctions play an important role in modulating Notch activity.
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影响因子：
10.5
作者：
ROSE, LS;WIESCHAUS, E
通讯作者：
WIESCHAUS, E
Sumio Nishikawa：“粘附连接蛋白沿着大鼠门牙分泌性成釉细胞之间可能的滑动界面定位。”
DOI：
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发表时间：
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影响因子：
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作者：
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Delta/Notch 信号传导参与斑马鱼成体色素条纹图案
影响因子：
4.6
作者：
H. Hamada;Masakatsu Watanabe;H. Lau;T. Nishida;T. Hasegawa;D. Parichy;Shigeru Kondo
通讯作者：
Shigeru Kondo
影响因子：
8.8
作者：
Khait, Itzhak;Orsher, Yuval;Sprinzak, David
通讯作者：
Sprinzak, David
DOI：
--
发表时间：
2013
期刊：
--
影响因子：
--
作者：
Hernan G. Garcia;Mikhail Tikhonov;A. Lin;Thomas Gregor
通讯作者：
Hernan G. Garcia;Mikhail Tikhonov;A. Lin;Thomas Gregor