A chinese herbal decoction prepared from radix astragali and radix angelicae sinensis induces the expression of erythropoietin in cultured Hep3B cells
A chinese herbal decoction prepared from radix astragali and radix angelicae sinensis induces the expression of erythropoietin in cultured Hep3B cells
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DOI：
10.1055/s-2008-1034322
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发表时间：
2008-03-01
期刊：
影响因子：
2.7
通讯作者：
Tsim, Karl W. K.
中科院分区：
文献类型：
--
作者：
Gao, Qiu T.;Cheung, Jerry K. H.;Tsim, Karl W. K.
当归补血汤（DBT）是一种常用的治疗妇女疾病的中药汤剂，含有黄芪（RA）和黄芪（RAS）。根据中医理论，这种汤是滋养血液功能;然而，这还没有在分子水平上得到证明。为了揭示该汤剂的造血作用，将DBT应用于培养的Hep 3B人肝癌细胞。DBT处理以剂量依赖性方式诱导促红细胞生成素（EPO）的mRNA表达，并在2.5倍诱导时达到峰值。通过ELISA定量培养的Hep 3B细胞中分泌的EPO：DBT处理增强了缺氧诱导的培养细胞中EPO表达的效果。此外，DBT诱导的EPO表达可被丝裂原活化激酶抑制剂U 0126预处理所消除。目前的结果验证了这种古老的草药汤剂的造血功能。
Danggui Buxue Tang (DBT), a Chinese medicinal decoction used commonly for treating women's ailments, contains Radix Astragali (RA) and Radix Angelicae Sinensis (RAS). According to Chinese medicinal theory, this decoction is to nourish the blood function; this, however, has not been demonstrated on the molecular level. In order to reveal the hematopoietic effect of this decoction, DBT was applied to cultured Hep3B human hepatocellular carcinoma cells. The treatment of DBT induced mRNA expression of erythropoietin (EPO) in a dose-dependent manner and peaked at similar to 2.5-fold induction. The secreted EPO in cultured Hep3B cells was quantified by ELISA: the treatment of DBT potentiated the effect of hypoxia-induced EPO expression in the cultured cells. In addition, the DBT-induced EPO expression could be abolished by pre-treatment with U0126, a mitogen-activated kinase inhibitor. The current results verified the hematopoietic function of this ancient herbal decoction.