In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method
In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method
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DOI：
10.1590/s0036-46652009000100002
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发表时间：
2009-02-01
期刊：
影响因子：
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通讯作者：
Silva, Maria do Rosário Rodrigues
中科院分区：
文献类型：
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作者：
Araújo, Crystiane Rodrigues;Miranda, Karla Carvalho;Silva, Maria do Rosário Rodrigues
用微量肉汤稀释法测定了氟康唑、伊曲康唑、酮康唑、特比奈芬和灰黄霉素对60株从巴西哥尼亚市患者指甲或皮肤标本中分离的皮肤真菌的抗真菌活性。在这项研究中，微滴定板在28摄氏度下培养，允许在培养4天后读取最小抑菌浓度(MIC)，对红色毛霉菌和犬小孢子菌培养5天后读出最小抑菌浓度(MIC)。大多数皮肤真菌对所测试的抗真菌药物的敏感性都是一致的。伊曲康唑、酮康唑和特比奈芬的MIC值分别为33.3%、31.6%和15%。
The antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from Goiania city patients, Brazil. In this study, the microtiter plates were incubated at 28 degrees C allowing a reading of the minimal inhibitory concentration ( MIC) after four days of incubation for Trichophyton mentagrophytes and five days for T. rubrum and Microsporum canis. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 0.03 mu g/mL were found for 33.3%, 31.6% and 15% of isolates for itraconazole, ketoconazole and terbinafine, respectively.