Genome-wide identification, evolution, and mRNA expression of complement genes in common carp (Cyprinus carpio)
Genome-wide identification, evolution, and mRNA expression of complement genes in common carp (Cyprinus carpio)
复制标题
鲤鱼 (Cyprinus carpio) 补体基因的全基因组鉴定、进化和 mRNA 表达。
DOI：
10.1016/j.fsi.2019.11.032
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发表时间：
2020-01-01
影响因子：
4.7
通讯作者：
Xu, Peng
中科院分区：
文献类型：
--
作者：
Lv, Hongzao;Zhou, Tao;Xu, Peng
补体是先天免疫系统的复杂组成部分，在抵御病原体和维持宿主体内平衡方面发挥着重要作用。补体系统在哺乳动物中已经得到了广泛的研究，但对硬骨鱼中的补体，尤其是四倍体鲤鱼的补体却知之甚少。本研究共鉴定和鉴定了110个鲤鱼补体基因，几乎囊括了哺乳动物补体基因的所有同源基因。这些基因被分为三种途径(替代途径、凝集素途径和经典途径)，与哺乳动物中的类似。系统发育和选择压力分析表明，补体基因进化受限，功能保守。在供试的12种鲤鱼健康组织中，大部分补体基因在脾、肝、脑和皮肤中高度表达。鲤鱼感染嗜水气单胞菌后，补体基因的许多成员被激活，产生免疫应答，并表达出来抵抗任何病原体的侵袭。两个同源基因之间的基因表达差异提示4R WGD事件后两个同源基因的功能差异，揭示了四倍体鲤鱼在最近WGD事件后的进化命运。
Complement is a complex component of innate immune system, playing an important role in defense against pathogens and host homeostasis. The complement system has been comprehensively studied in mammals, however less is known about complement in teleost, especially in tetraploid common carp (Cyprinus carpio). In this study, a total of 110 complement genes were identified and characterized in common carp, which include almost all the homologs of mammalian complement genes. These genes were classified into three pathways (alternative pathways, lectin pathways and classical pathways), similar to those in mammals. Phylogenetic and selection pressure analysis showed that the complement genes were evolving-constrained and the function was conserved. Most of the complement genes were highly expressed in spleen, liver, brain and skin among the tested 12 health tissues of common carp. After Aeromonas hydrophila infection in the common carp, many members of complement genes were activated to bring about an immune response and expressed to against any pathogenic encroachment. Gene expression divergences which were found between two homoeologous genes suggested the functional divergences of the homoeologous genes after the 4R WGD event, revealing the evolutionary fate of the tetraploid common carp after the recent WGD.