Interleukin-8 in the human endometrium.
Interleukin-8 in the human endometrium.
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DOI：
10.1210/jcem.83.5.4754
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发表时间：
1998-05
期刊：
影响因子：
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通讯作者：
Aydin Arici;E. Seli;Levent M. Senturk;Linda S. Gutierrez;Engin Oral;Hugh S. Taylor
中科院分区：
文献类型：
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作者：
Aydin Arici;E. Seli;Levent M. Senturk;Linda S. Gutierrez;Engin Oral;Hugh S. Taylor
我们之前已经证明，白介素 8 (IL-8) 是一种具有中性粒细胞趋化/激活和 T 细胞趋化活性的细胞因子，是由培养的人子宫内膜基质和腺细胞产生的。本研究调查了人子宫内膜中 IL-8 信使核糖核酸 (mRNA) 和蛋白质的时空表达。子宫内膜组织 (n = 52) 是在因子宫内膜疾病以外的原因进行子宫切除术后从人类子宫或子宫内膜活检中获得的。月经周期的日期是根据女性的月经史确定的，并通过组织学证实。将一半组织 (n = 26) 在液氮中速冻，提取细胞 RNA，并将 Northern 印迹与与 IL-8 mRNA 特定序列互补的核糖探针杂交。其余组织 (n = 26) 进行冷冻切片处理，并使用小鼠抗人 IL-8 抗体进行免疫组织化学分析。整个月经周期IL-8 mRNA水平的比较显示，分泌晚期和增殖早中期IL-8表达显着高于周期中期表达（P < 0.02）。 IL-8 免疫组织化学分析表明，IL-8 蛋白存在于整个月经周期的表面上皮和腺体中。基质细胞中未检测到免疫反应性 IL-8。我们得出结论，IL-8是在体内人类子宫内膜中产生的，并且IL-8 mRNA在整个月经周期中的变化表明性激素可能调节其基因表达。我们推测 IL-8 可能调节中性粒细胞和淋巴细胞及时募集到子宫内膜中。
We have previously shown that interleukin-8 (IL-8), a cytokine with neutrophil chemotactic/activating and T cell chemotactic activity, is produced by human endometrial stromal and glandular cells in culture. The present study investigated the temporal and spatial expression of IL-8 messenger ribonucleic acid (mRNA) and protein in the human endometrium. Endometrial tissue (n = 52) was obtained from human uteri after hysterectomy conducted for reasons other than endometrial disease or from endometrial biopsies. The day of the menstrual cycle was established from women's menstrual history and was confirmed by histology. Half of the tissues (n = 26) were snap-frozen in liquid nitrogen, cellular RNA was extracted, and Northern blots were hybridized with a riboprobe complementary to a specific sequence of IL-8 mRNA. The remaining tissues (n = 26) were processed for frozen sections, and immunohistochemistry was performed using mouse antihuman IL-8 antibody. Comparison of IL-8 mRNA levels throughout the menstrual cycle revealed that late secretory and early to midproliferative phase IL-8 expression was significantly greater than midcycle expression (P < 0.02). Analysis of the IL-8 immunohistochemistry revealed that IL-8 protein is found in the surface epithelium and glands throughout the menstrual cycle. There was no detectable immunoreactive IL-8 in the stromal cells. We conclude that IL-8 is produced in the human endometrium in vivo, and the variations of IL-8 mRNA throughout the menstrual cycle suggest that sex hormones may regulate its gene expression. We speculate that IL-8 may modulate the timely recruitment of neutrophils and lymphocytes into the endometrium.