MECHANICAL REGULATION OF CARTILAGE BIOSYNTHETIC BEHAVIOR - PHYSICAL STIMULI
MECHANICAL REGULATION OF CARTILAGE BIOSYNTHETIC BEHAVIOR - PHYSICAL STIMULI
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DOI：
10.1006/abbi.1994.1201
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发表时间：
1994-05-15
影响因子：
3.9
通讯作者：
SANDY, JD
中科院分区：
文献类型：
--
作者：
KIM, YJ;SAH, RLY;SANDY, JD
研究了小牛软骨盘外植体在宽频率范围的径向无侧限压缩下对小振幅动态压缩的生物合成反应。振荡流体流动，静水压力，流动电位，和细胞变形在调节软骨细胞代谢的相对重要性进行了探讨，通过量化的频率依赖性和空间（径向）分布的生物合成反应内的3毫米直径外植体磁盘。在大于0.001 Hz（周期/秒）的频率下，动态压缩增加了蛋白聚糖和蛋白质的生物合成。虽然在0.002和0.01 Hz之间的频率压缩引起的生物合成的刺激，分布在整个磁盘，压缩在0.1 Hz引起的刺激，主要局限于外部径向周边。这些分布进行了比较，以前的估计径向分布的物理力和流动的矩阵内。结果表明，在振幅高达10%（应力高达0.5 MPa）的动态机械压缩的软骨细胞生物合成的刺激有关的流体流动和/或细胞形状的变化，而不是流体静压力的变化。由于静态压缩到原来的切割厚度引起的生物合成在光盘的中心略有下降，我们还研究了有限的扩散运输在显着抑制合成过程中看到的大位移静态压缩的可能作用。实验中，磁盘的表面积与体积比或标记底物或血清的浓度是不同的，没有证据表明，有限的扩散运输是负责大位移静态压缩的生物合成的抑制。从静态压缩和压缩组织的组织学分析的生物合成的恢复表明，即使在12小时的50%静态压缩期间，也没有显著的细胞损伤。(C)1994年出版社出版。
The biosynthetic response of calf cartilage disk explants to small-amplitude dynamic compression was studied in radially unconfined compression over a wide range of frequencies. The relative importance of oscillatory fluid flow, hydrostatic pressure, streaming potential, and cell deformation in modulating chondrocyte metabolism was explored by quantifying the frequency dependence and the spatial (radial) distribution of the biosynthetic response within the 3-mm-diameter explant disks. At frequencies greater than 0.001 Hz (cycle/s), dynamic compression increased biosynthesis of proteoglycans and proteins. While compression at frequencies between 0.002 and 0.01 Hz caused a stimulation of biosynthesis that was distributed throughout the disk, compression at 0.1 Hz caused a stimulation that was confined mainly to the outer radial periphery. These distributions were compared to previous estimates of the radial distribution of physical forces and flows within the matrix. The results suggest that the stimulation of chondrocyte biosynthesis by dynamic mechanical compression at amplitudes up to 10% (stresses up to 0.5 MPa) is related to changes in fluid flow and/or cell shape rather than changes in hydrostatic pressure. Since static compression to the original cut thickness caused a slight decrease in biosynthesis in the center of the disks, we also studied the possible role of limited diffusive transport in the marked inhibition of synthesis seen during large displacement static compression. Experiments in which the surface area-to-volume ratio of disks or the concentration of labeling substrate or serum were varied provided no evidence that limited diffusive transport was responsible for the inhibition of biosynthesis by large displacement static compression. Recovery of biosynthesis from static compression and histological analyses of compressed tissue suggested that there was no significant cell damage even during 12 h of 50% static compression. (C) 1994 Academic Press, Inc.