Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
复制标题
鉴定和纯化人免疫球蛋白增强子结合蛋白 (NF-kappa B)，该蛋白可在体外激活人类免疫缺陷病毒 1 型启动子的转录。
DOI：
10.1073/pnas.85.13.4700
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发表时间：
1988
影响因子：
11.1
通讯作者：
Roeder,RG
中科院分区：
文献类型：
--
作者：
Kawakami,K;Scheidereit,C;Roeder,RG
增强子结合因子NF-kappa B仅存在于转录免疫球蛋白轻链基因的细胞中，通过序列特异性DNA亲和层析从Namalwa细胞（人伯基特淋巴瘤细胞）的核提取物中纯化得到。经紫外交联鉴定，纯化的NF-kappa B为51 kda多肽。“足迹”和甲基化干扰分析表明，纯化的NF-kappa B具有kappa轻链增强子序列特异性的结合活性。纯化的因子通过与上游nf - κ b结合位点结合，激活了人类免疫缺陷病毒I型启动子的体外转录。
The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polypeptide by UV-crosslinking analysis. "Footprint" and methylation-interference analyses have shown that purified NF-kappa B has a binding activity specific for the kappa light chain enhancer sequence. The purified factor activated in vitro transcription of the human immunodeficiency virus type I promoter by binding to an upstream NF-kappa B-binding site.