Schistosoma mansoni excretory-secretory products stimulate a p38 signalling pathway in Biomphalaria glabrata embryonic cells
Schistosoma mansoni excretory-secretory products stimulate a p38 signalling pathway in Biomphalaria glabrata embryonic cells
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DOI：
10.1016/j.ijpara.2005.08.009
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发表时间：
2006-01-01
影响因子：
4
通讯作者：
Yoshino, TP
中科院分区：
文献类型：
--
作者：
Humphries, JE;Yoshino, TP
感染曼氏血吸虫幼虫后，抗性光滑双脐螺的血细胞执行快速防御，在此期间，它们向寄生虫迁移并包裹寄生虫。这种即时和精确的反应被认为取决于信号转导级联反应，虽然所涉及的信号成分仍然在很大程度上未知。有丝分裂原活化蛋白激酶可能在B中起作用。glabrata免疫信号传导，特别是p38丝裂原活化蛋白激酶，已知其与应激和炎症信号传导相关。使用简并PCR，然后快速扩增cDNA末端，从两个B中克隆了全长p38有丝分裂原活化蛋白激酶样cDNA。光滑胚（Bge）细胞系（Bge-p38）和血细胞（Bgh-p38）。此外，B.使用特异性识别活化的/二磷酸化的p38促分裂原活化蛋白激酶的抗体，在蛋白质印迹分析中在蛋白质水平上检测光滑的p38促分裂原活化蛋白激酶的活化。结果表明，Bge细胞p38丝裂原活化蛋白激酶被激活/磷酸化后30分钟的茴香霉素，建立p38丝裂原活化蛋白激酶激活剂温育。此外，p38丝裂原活化蛋白激酶也在暴露于β-葡聚糖聚合物海带多糖或S.曼氏幼虫的排泄分泌产物。在一项比较研究中，激活的血细胞p38丝裂原活化蛋白激酶也可以检测到使用抗磷酸化p38抗体后，细胞处理与茴香霉素。然而，与BGE细胞相比，血细胞p38没有被激活的排泄分泌产物或海带多糖治疗，这表明血细胞和BGE细胞之间的信号转导途径中的p38丝裂原活化蛋白激酶的作用的根本差异。(c)2005年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Following infection with Schistosoma mansoni larvae, haemocytes of resistant Biomphalaria glabrata snails execute a rapid defence during which they migrate towards and encapsulate the parasites. Such immediate and precise responses are thought to depend on signal transduction cascades though the signalling components involved remain largely unknown. It is proposed that mitogen-activated protein kinases may play a role in B. glabrata immune signalling, in particular p38 mitogen-activated protein kinases, which are known to be associated with stress and inflammatory signalling. Using degenerate PCR followed by Rapid Amplification of cDNA Ends a full-length p38 mitogen-activated protein kinase-like cDNA was cloned from both the B. glabrata embryonic (Bge) cell line (Bge-p38) and haemocytes (Bgh-p38). In addition, B. glabrata p38 mitogen-activated protein kinase activation was examined at the protein level in Western blot analyses using an antibody that specifically recognises activated/diphosphorylated p38 mitogen-activated protein kinase. Results showed that Bge cell p38 mitogen-activated protein kinase was activated/phosphorylated following 30 min incubation with anisomycin, an established p38 mitogen-activated protein kinase activator. Further-more, p38 mitogen-activated protein kinase was also activated after only 5 min exposure to either the beta-glucan polymer laminarin or S. mansoni larval excretory-secretory products. In a comparative study, activated haemocyte p38 mitogen-activated protein kinase could also be detected using the anti-phosphorylated p38 antibody following cell treatment with anisomycin. However, in contrast with Bge cells, haemocyte p38 was not activated by either excretory-secretory products or laminarin treatments, suggesting fundamental differences in the role of p38 mitogen-activated protein kinase in signal transduction pathways between haemocytes and Bge cells. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.