A gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer.
A gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer.
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DOI：
10.1016/j.ijpharm.2010.06.012
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发表时间：
2010-08
影响因子：
5.8
通讯作者：
Jun Oishi;Joohee Jung;A. Tsuchiya;R. Toita;Jeong-Hun Kang;Takeshi Mori;T. Niidome;K. Tanizawa
中科院分区：
文献类型：
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作者：
Jun Oishi;Joohee Jung;A. Tsuchiya;R. Toita;Jeong-Hun Kang;Takeshi Mori;T. Niidome;K. Tanizawa
最近，我们的小组提出了一种响应 cAMP 依赖性蛋白激酶 (PKA) 的新型基因调控系统，该系统已应用于活细胞。在这项研究中，人类肝脏特异性生物纳米胶囊（BNC）被用作基因传递系统，以提高转染效率并靶向特定细胞类型。 BNCs可以在体外或体内有效地将靶基因递送至人肝细胞和肝癌细胞。信号响应基因传递系统与 BNC 的结合提高了肝癌细胞的转染效率和选择性。所递送基因的表达在 PKA 激活的肝癌细胞 (HepG2) 中得到鉴定，但在结肠肿瘤细胞 (WiDr) 中没有得到鉴定。这些结果表明，响应细胞内信号的基因调控系统与BNC的组合可用于人肝癌细胞的选择性治疗。
Recently, our group has proposed a novel gene-regulation system responding to cAMP-dependent protein kinase (PKA) that has been applied to living cells. In this study, human liver-specific bionanocapsules (BNCs) are used as a gene-delivery system to increase transfection efficiency and to target specific cell types. BNCs can efficiently deliver a target gene to human hepatocytes and hepatoma cells in vitro or in vivo. The combination of a signal-responsive gene-delivery system with BNCs led to an increase in the transfection efficiency and selectivity for hepatoma cells. Expression from the delivered gene was identified from PKA-activated hepatoma cells (HepG2), but not from colon tumor cells (WiDr). These results show that the combination of a gene-regulation system responding to an intracellular signal with BNC can be used for the selective treatment of human hepatoma cells.