Intraspecies variability of cellular fatty acids among soil and intestinal strains of Desulfovibrio desulfuricans
Intraspecies variability of cellular fatty acids among soil and intestinal strains of Desulfovibrio desulfuricans
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DOI：
10.1128/aem.62.9.3360-3365.1996
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发表时间：
1996-09-01
影响因子：
4.4
通讯作者：
Wilczok, T
中科院分区：
文献类型：
--
作者：
Dzierzewicz, Z;Cwalina, B;Wilczok, T
对从土壤和人体肠道两种完全不同的环境中分离的脱硫弧菌DSM 642和该种的14株野生菌株的细胞脂肪酸组成进行了比较。结果表明，在乳酸盐和硫酸盐上生长的脱硫弧菌均含有相当数量的I-C-15：0、I-C-17：1和C-16：0。虽然土壤菌株组中各菌株的脂肪酸组成有明显的差异(相似性为67.6%)，而肠道菌株的脂肪酸图谱几乎相同(相似性接近100%)，但结果在物种论证可接受的范围内是不同的，肠道菌株脂肪酸图谱的相似性较高可能是由于人类消化道中的生物群系相似所致。I-C-17：1和I-C-15：0(主要支链脂肪酸)的变异系数，以及在绘制I-C-17：1脂肪酸与I-C-15：0脂肪酸的百分比后对调查菌株与文献中描述的菌株进行的聚类，证实了土壤菌株组内细胞脂肪酸谱的一定异质性，而不是肠道分离物组内的几乎理想的同质性。肠道菌株的饱和/不饱和脂肪酸比值(2.2+/-0.14)高于土壤菌株(1.6+/-0.2；1例为2.7)，我们认为肠道脱硫弧菌应被认为是一个高度同质性的类群，在微生物培养群中应以脱硫弧菌亚种肠杆菌为代表。
A comparison of cellular fatty acid profiles of Desulfovibrio desulfuricans DSM 642 and 14 wild strains of this species, isolated from two completely different environments, soil and the human intestine, was carried out, All the D. desulfuricans strains grown on lactate and sulfate indicated the presence of considerable amounts of i-C-15:0, i-C-17:1 and C-16:0. Although differences in the quantities of individual fatty acids present in each strain were clear in the group of soil strains (similarity, 67.6%), in contrast to almost identical fatty acid patterns (similarity, near 100%) in the intestinal strains, the results were variable within the limits acceptable for species demonstration, The higher similarity of the fatty acid profiles of intestinal strains may be a result of the similarity of biocenoses in the human digestive tract. The coefficients of variability of i-C-17:1 and i-C-15:0 (the major branched-chain fatty acids), as well as clustering of the investigated strains compared with strains described in the literature after plotting percentages of i-C-17:1 fatty acid against i-C-15:0 fatty acid, confirmed a certain heterogeneity of cellular fatty acid profiles within the group of soil strains, in contrast to almost ideal homogeneity within the group of intestinal isolates. Intestinal strains contained a higher ratio of saturated to unsaturated fatty acids (2.2 +/- 0.14) than did soil strains (1.6 +/- 0.2; in one case, 2.7), We propose that intestinal D. desulfovibrio bacteria should be assumed to be a highly homogeneous group and should be represented by the strain D. desulfuricans subsp, intestinus in collections of microbial cultures.