EWS-FLI-1 AND EWS-ERG CHIMERIC MESSENGER-RNAS IN EWINGS-SARCOMA AND PRIMITIVE NEUROECTODERMAL TUMOR
EWS-FLI-1 AND EWS-ERG CHIMERIC MESSENGER-RNAS IN EWINGS-SARCOMA AND PRIMITIVE NEUROECTODERMAL TUMOR
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DOI：
10.1002/ijc.2910630407
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发表时间：
1995-11-15
影响因子：
6.4
通讯作者：
HAYASHI, Y
中科院分区：
文献类型：
--
作者：
IDA, K;KOBAYASHI, S;HAYASHI, Y
t（11; 22）（q24; q12）和t（21; 22）（q22; q12）是在尤文家族肿瘤（包括ES、PNET和Askin肿瘤）中发现的特异性染色体易位。在这些易位中，位于22 q12的EWS基因的氨基末端部分融合到位于11 q24的FLI-1基因或位于21 q22的ERG基因的羧基末端部分，它们属于转录激活因子的ets癌基因超家族。我们用RT-PCR和测序的方法研究了15个ES（7个细胞系和8个肿瘤样品）和7个PNIPs（3个细胞系和4个肿瘤样品）的嵌合mRNA。在2株ES细胞系和7例PNET肿瘤标本中检测到2种EWS-ERG嵌合体mRNA，在11株ES细胞（4株ES细胞系和7例PNET肿瘤标本）和4例PNET细胞（2株ES细胞系和2例PNET肿瘤标本）中检测到4种EWS-FLI-1嵌合体mRNA。嵌合体mRNA的类型与患者的性别、年龄、原发部位和组织病理学等临床特征之间似乎没有关联。所有的嵌合mRNA都是由框内连接产生的，并被认为编码融合蛋白，这可能是尤因家族肿瘤中涉及的分子机制。(C)1995年Wiley-Liss，Inc.
The t(11;22)(q24;q12) and t(21;22)(q22;q12) are specific chromosomal translocations found in the Ewing family of tumors including ES, PNET and Askin tumors. In these translocations, the amino-terminal portion of the EWS gene located in 22q12 fuses to the carboxyl-terminal portion of the FLI-1 gene located in 11q24 or the ERG gene located in 21q22, which belong to the ets oncogene superfamily of transcription activators. We investigated the chimeric mRNAs of 15 ESs (7 cell liner and 8 tumor samples) and 7 PNETs (3 cell lines and 4 tumor samples) using the RT-PCR method and sequencing. We detected 2 types of EWS-ERG chimeric mRNA in 2 ES cell lines and PNET tumor sample in addition to 4 types of EWS-FLI-1 chimeric mRNA in 11 ESs (4 cell lines and 7 tumor samples) and 4 PNETs (2 cell lines and 2 tumor samples). There seemed to be no association between the type of chimeric mRNA and clinical features such as sex, age, primary site and histopathology of the patients. All of the chimeric mRNAs are generated from in-frame junctions and are thought to encode fusion proteins that may be the molecular mechanism involved in the Ewing family of tumors. (C) 1995 Wiley-Liss, Inc.