Regulation of Recombinase Polymerase Amplification by Vibrational Strong Coupling of Water
Regulation of Recombinase Polymerase Amplification by Vibrational Strong Coupling of Water
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DOI：
10.1021/acsphotonics.3c00243
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发表时间：
2023-04-27
期刊：
影响因子：
7
通讯作者：
Zhang, Feng
中科院分区：
文献类型：
--
作者：
Gu, Kaihao;Si, Qiankang;Zhang, Feng
分子跃迁和光子结构之间的振动强耦合（VSC）可用于调节分子的反应性，并且混合极化子态已用于改变光学腔中分子系统的能量景观。为了研究如何用 VSC 调节生命反应，本文中我们选择了体外模型生物反应、DNA 复制，即重组酶聚合酶扩增 (RPA)，与典型的 Fabry-Pe ' rot (FP) 腔模式相结合。 RPA 被称为等温酶促反应，可以很好地满足 FP 腔内的 VSC 研究。通过调节水分子和 FP 腔模之间的共振耦合强度，我们可以在腔模从 3100 cm-1 变化到 3550 cm-1 时调节 RPA 的效率。结果表明，基于O-H的VSC可以显着抑制RPA的效率，约58%。由于水在生命中的关键作用，O-H伸缩振动与光子结构的耦合可能成为调节生命的新工具。
Vibrational strong coupling (VSC) between molecular transitions and photonic structures can be used to tune the reactivity of molecules, and hybrid polariton states have been employed to modify the energy landscape of molecular systems in an optical cavity. To investigate how to modulate life reactions with VSC, herein we selected an in vitro model bioreaction, DNA replication, i.e., recombinase polymerase amplification (RPA), to couple with the typical Fabry-Pe ' rot (FP) cavity mode. Known as an isothermal enzymatic reaction, RPA well satisfies the VSC study within FP cavities. Through tuning the resonance coupling strength between water molecules and FP cavity modes, we can regulate the efficiency of RPA as the cavity modes change from 3100 to 3550 cm-1. The results showed that O-H-based VSC can significantly inhibit the efficiency of RPA by similar to 58%. Due to the key role of water in life, O-H stretching vibrational coupling with photonic structures could be a novel tool to regulate life in turn.