Overexpression of profilin reduces the migration of invasive breast cancer cells
Overexpression of profilin reduces the migration of invasive breast cancer cells
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DOI：
10.1002/cm.10160
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发表时间：
2004-02-01
影响因子：
--
通讯作者：
Jacobson, K
中科院分区：
文献类型：
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作者：
Roy, P;Jacobson, K
profilin在细胞迁移中的确切作用尚不清楚。在这项研究中，我们评估了profilin的过度表达对乳腺癌细胞迁移的影响。通过在BT474细胞中稳定表达GFP-profilin进行过表达。观察到，即使是中等水平的profilin过表达也显著损害了BT474细胞在纤连蛋白包被的基底上扩散和响应EGF而迁移的能力。与亲本和GFP表达（对照）细胞微粒相比，GFP-profilin表达细胞还显示出响应于胰蛋白酶的增加的对脱离的抗性以及粘着斑激酶（FAK）和桩蛋白的增加的酪氨酸磷酸化。这些结果表明，干扰profilin水平可能提供了一个很好的策略，用于控制乳腺癌细胞的转移潜力。(C)2004 Wiley-Liss，Inc.
The exact role profilin plays in cell migration is not clear. In this study, we have evaluated the effect of overexpression of profilin on the migration of breast cancer cells. Overexpression was carried out by stably expressing GFP-profilin in BT474 cells. It was observed that even a moderate level of overexpression of profilin significantly impaired the ability of BT474 cells to spread on fibronectin-coated substrate and migrate in response to EGF. GFP-profilin expressing cells also showed increased resistance to detachment in response to trypsin and increased tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin compared to the parental and GFP-expressing (control) cell fines. These results suggest that perturbation of profilin levels may offer a good strategy for controlling the metastatic potential of breast cancer cells. (C) 2004 Wiley-Liss, Inc.