Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells
Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells
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DOI：
10.1007/s11626-014-9860-0
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发表时间：
2015-05-01
影响因子：
2.1
通讯作者：
Ohneda, Osamu
中科院分区：
文献类型：
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作者：
Sato, Kazutoshi;Itoh, Takehiro;Ohneda, Osamu
使用人骨髓间充质干细胞(MSCs)进行细胞治疗是治疗许多难治性疾病的一种有吸引力的方法。脂肪组织来源的间充质干细胞(AT-MSCs)因其在体内含量丰富、易于增殖、细胞因子产生能力高而被认为是一种良好的工具。为了避免在培养中使用胎牛血清(FBS)的相关风险，包括批次变异和病原体污染，无血清培养系统的开发已经开始。我们研制了一种完全无血清的培养液(SFM)，不仅可以用于AT-MSCs的扩增，还可以用于初步分离。在无血清条件下分离培养的AT-MSCs(AT-MSCs/SFM)在体外具有较高的增殖能力和向成骨细胞、脂肪细胞和软骨细胞分化的潜能。在体内骨分数模型分析中，AT-MSCs/SFM显示出比含血清培养的细胞(AT-MSCs/SCM)更高的骨修复能力和再生骨质量。这归因于(I)被照亮的翻译细胞的体内成像所证明的在骨中存在翻译细胞，以及(Ii)AT-MSCs/SFM对细胞因子BMP2、CCL2和CCL5的高水平表达和诱导能力。综上所述，我们报道了一种新的适用于细胞治疗的AT-MSCs无血清培养体系。
Cell therapy using human mesenchymal stem cells (MSCs) is an attractive approach for many refractory diseases. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are considered as a favorable tool due to its abundance in the body, easy proliferation, and high cytokine production potency. In order to avoid the risks associated with the use of fetal bovine serum (FBS) in culture that includes batch variations and contamination with pathogens, development of serum-free culture system has been initiated. We have formulated a completely serum-free culture medium (SFM) that could be used not only for the expansion of AT-MSCs but also for initial isolation. We demonstrate that the AT-MSCs isolated and cultured in serum-free medium (AT-MSCs/SFM) possess high proliferation capacity and differentiation potency to osteoblast, adipocyte, and chondrocyte lineages in vitro. In in vivo bone fraction model analysis, AT-MSCs/SFM showed higher bone repair potency and quality of the regenerated bone than the cells cultured in serum-containing medium (AT-MSCs/SCM). This was attributed to the (i) presence of translated cells in the bone, as evidenced by in vivo imaging of the illuminated translated cells and (ii) high level of expression and induction capacity of AT-MSCs/SFM for cytokine BMP2, CCL2, and CCL5. Taken together, we report a new serum-free culture system for AT-MSCs that is suitable for cell therapy.