Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils.
Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils.
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ADAMTS 蛋白酶和 ADAMTS 样蛋白与原纤维蛋白微纤维的基于细胞的相互作用分析。
DOI：
10.1007/978-1-4939-9698-8_16
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Hubmacher,Dirk
中科院分区：
文献类型：
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作者：
Hubmacher,Dirk
细胞外基质（ECM）是一种复合生物材料，可作为细胞的锚，并为细胞迁移、增殖和分化提供指导线索。然而，许多细节的分层ECM组装过程和个别蛋白质-蛋白质相互作用的作用还没有得到很好的理解。在这里，我描述了一种基于细胞培养的方法，允许确定的ECM定位的重组ADAMTS蛋白酶和ADAMTS样（L）蛋白的关系，在沉积的人皮肤成纤维细胞的微纤维中。该方法可以很容易地适用于研究定位的ECM组件以外的ADAMTS和ADAMTSL蛋白质的微纤维和其他ECM网络中的细胞外基质。
The extracellular matrix (ECM) is a composite biomaterial that serves as an anchor for cells and provides guidance cues for cell migration, proliferation, and differentiation. However, many details of the hierarchical ECM assembly process and the role of individual protein–protein interactions are not well understood. Here, I describe a cell-culture-based method that allows for determination of the ECM localization of recombinant ADAMTS proteases and ADAMTS-like (L) proteins in relationship to fibrillin microfibrils deposited by human dermal fibroblasts. The method can be readily adapted to study the localization of ECM components other than ADAMTS and ADAMTSL proteins to fibrillin microfibrils and other ECM networks.