Infrequent Detection of KI, WU and MC Polyomaviruses in Immunosuppressed Individuals with or without Progressive Multifocal Leukoencephalopathy
Infrequent Detection of KI, WU and MC Polyomaviruses in Immunosuppressed Individuals with or without Progressive Multifocal Leukoencephalopathy
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DOI：
10.1371/journal.pone.0016736
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发表时间：
2011-03-16
期刊：
影响因子：
3.7
通讯作者：
Tan, Chen S.
中科院分区：
文献类型：
--
作者：
Dang, Xin;Bialasiewicz, Seweryn;Tan, Chen S.
据报道，在进行性多灶性白质脑病 (PML) 患者样本中，新发现的 KI(KIPyV)、WU(WUPyV) 和默克尔细胞癌 (MCPyV) 多瘤病毒的患病率存在冲突，范围为 0% 至 14.3%。我们分析了这些多瘤病毒在 PML 患者、患有或不患有 HIV 的免疫抑制个体以及多发性硬化症 (MS) 患者的脑脊液 (CSF)、外周血单核细胞 (PBMC) 和骨髓样本中的流行情况。两个独立实验室对 KIPyV、WUPyV 和 MCPyV DNA 进行的不同 PCR 测试仅在 1/269 个样本中检测到低水平的 MCPyV DNA。在免疫抑制个体（包括患有 PML 的个体）的多个样本中很少检测到这些病毒，表明它们的再激活机制可能与 JC 多瘤病毒 (JCPyV) 不同，并且它们在 PML 的发病机制中不发挥作用。
Conflicting prevalence of newly identified KI(KIPyV), WU(WUPyV) and Merkel Cell Carcinoma(MCPyV) polyomaviruses have been reported in progressive multifocal leukoencephalopathy(PML) patient samples, ranging from 0 to 14.3%. We analyzed the prevalence of these polyomaviruses in cerebrospinal fluid(CSF), peripheral blood mononuclear cells(PBMC), and bone marrow samples from PML patients, immunosuppressed individuals with or without HIV, and multiple sclerosis(MS) patients. Distinct PCR tests for KIPyV, WUPyV and MCPyV DNA performed in two independent laboratories detected low levels of MCPyV DNA only in 1/269 samples. The infrequent detections of these viruses in multiple samples from immunosuppressed individuals including those with PML suggest that their reactivation mechanisms may be different from that of JC polyomavirus (JCPyV) and that they do not play a role in the pathogenesis of PML.