Genetic linkage map construction and QTL mapping for yield and fiber quality in upland cotton (Gossypium hirsutum L.).
Genetic linkage map construction and QTL mapping for yield and fiber quality in upland cotton (Gossypium hirsutum L.).
复制标题
DOI：
10.3724/sp.j.1006.2008.01199
复制
发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Ma, J.
中科院分区：
文献类型：
--
作者：
Chen Li, Chen Li;Zhang ZhengSheng, Zhang ZhengSheng;Ma, J.
利用3458对SSR引物对中35和豫棉1号进行多态性引物筛选，得到173对多态性引物。利用所设计的多态性引物对中35 ×豫棉1号F2群体的180个单株进行基因型分析，共获得178个位点。构建了一张包含148个SSR位点和36个连锁群的遗传连锁图谱，图谱全长1309.2cM，标记间平均间隔8.8cM，占棉花基因组的29.5%。36个连锁群中有28个位于20条染色体上，8个不位于任何染色体上。利用180个F2代单株和F2：3家系的产量和纤维品质数据，通过区间作图法检测了9个控制产量和纤维品质的QTL，其中衣分2个，铃重1个，籽指1个，纤维长度1个，纤维强度2个，纤维细度2个。其中LP 1、BW、SD、FL和FS 1 5个QTL位于第7染色体上，LP 2、FS 2、FF 1和FF 2 4个QTL分别位于第15、21、9和20染色体上。5个纤维品质QTL的有利等位基因均来自豫棉1号。
A total of 3458 SSR primer pairs were used to screen the polymorphic primers between cotton genotypes Zhong 35 and Yumian 1, and 173 polymorphic primer pairs were obtained. The polymorphic primer pairs were used to genotype the 180 individual plants of F2 population derived from (Zhong 35 * Yumian 1), and 178 loci were obtained. A genetic linkage map with 148 SSR loci and 36 linkage groups was constructed, and the linkage map covered a whole length of 1309.2 cM with an average interval of 8.8 cM between two markers, accounting for 29.5% of the cotton genome. Twenty-eight out of 36 linkage groups were located on 20 chromosomes, and 8 linkage groups were not located on any chromosomes. The data of yield and fibre qualities of the 180 F2 plants and F2:3 family lines were used to detect QTLs by interval mapping, and 9 QTLs controlling yield and fibre quality were identified, including two QTLs for lint percent (LP), one for boll weight (BW), one for seed index (SD), one for fibre length (FL), two for fibre strength (FS), and two for fibre fineness (FF). The five QTLs, LP1, BW, SD, FL, and FS1, were located on chromosome 7, and the other four QTLs, LP2, FS2, FF1, and FF2, on chromosomes 15, 21, 9, and 20, respectively. The favourable alleles of all five QTLs for fibre qualities originated from Yumian 1.