Polyethylene and titanium particles induce osteolysis by similar, lymphocyte-independent, mechanisms
Polyethylene and titanium particles induce osteolysis by similar, lymphocyte-independent, mechanisms
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DOI：
10.1016/j.orthres.2004.08.023
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发表时间：
2005-03-01
影响因子：
2.8
通讯作者：
Greenfield, EM
中科院分区：
文献类型：
--
作者：
Taki, N;Tatro, JM;Greenfield, EM
假体周围骨溶解是限制全关节置换术患者长期生存的主要临床问题。骨溶解是由植入物产生的磨损颗粒引起的，主要来自聚乙烯承载表面。这项研究检验了两个假设。首先，聚乙烯和钛颗粒诱导的骨溶解也有类似的机制。第二，淋巴细胞在颗粒诱导的骨溶解中不起主要作用。为了验证这些假设，我们使用了先前用于检查钛诱导的骨溶解的小鼠颅骨模型。聚乙烯颗粒在植入后5-7天迅速诱导小鼠颅骨骨溶解。聚乙烯诱导的骨溶解与大量破骨细胞以及厚骨膜纤维组织层的形成有关，其中大量巨噬细胞含有被吞噬的聚乙烯颗粒。聚乙烯诱导的骨溶解迅速修复，并在植入后第21天检测不到。野生型小鼠纤维层可见淋巴细胞。然而，在Pfp/Rag2双敲除小鼠中，聚乙烯颗粒诱导的骨溶解量和细胞因子的产生并未受到淋巴细胞缺乏的实质性影响。所有这些发现都与我们观察到的钛颗粒引起的骨溶解相似。这些结果为我们的两个假设提供了强有力的支持：聚乙烯和钛颗粒诱导的骨溶解有相似的机制，淋巴细胞在颗粒诱导的骨溶解中不起主要作用。(c) 2004年骨科研究学会。Elsevier Ltd.出版。版权所有。
Periprosthetic osteolysis is a major clinical problem that limits the long-term survival of total joint arthroplasties. Osteolysis is induced by implant-derived wear particles, primarily from the polyethylene bearing surfaces. This study examined two hypotheses. First, that similar mechanisms are responsible for osteolysis induced by polyethylene and titanium particles. Second, that lymphocytes do not play a major role in particle-induced osteolysis. To test these hypotheses, we used the murine calvarial model that we have previously used to examine titanium-induced osteolysis. Polyethylene particles rapidly induced osteolysis in the murine calvaria 5-7 days after implantation. The polyethylene-induced osteolysis was associated with large numbers of osteoclasts as well as the formation of a thick periosteal fibrous tissue layer with numerous macrophages containing phagocytosed polyethylene particles. Polyethylene-induced osteolysis was rapidly repaired and was undetectable by day 21 after implantation. Lymphocytes were noted in the fibrous layer of wild-type mice. However, the amount of osteolysis and cytokine production induced by polyethylene particles was not substantially affected by the lack of lymphocytes in Pfp/Rag2 double knock out mice. All of these findings are similar to our observations of osteolysis induced by titanium particles. These results provide strong support for both of our hypotheses: that similar mechanisms are responsible for osteolysis induced by polyethylene and titanium particles and that lymphocytes do not play a major role in particle-induced osteolysis. (c) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.