Three distinct families of GABAergic neurons in rat visual cortex
Three distinct families of GABAergic neurons in rat visual cortex
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DOI：
10.1093/cercor/7.4.347
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发表时间：
1997-06-01
期刊：
影响因子：
3.7
通讯作者：
Burkhalter, A
中科院分区：
文献类型：
--
作者：
Gonchar, Y;Burkhalter, A
在大脑皮层，抑制主要由GABA能中间神经元介导。尽管所有这些神经元使用相同的神经递质，但对大鼠额叶皮质的研究表明，它们在分子和生理上是不同的。目前尚不清楚初级视皮层中是否存在类似的GABA能神经元亚群，以及这些不同的抑制性神经元是如何插入特定的皮质回路的。我们使用了针对伽马氨基丁酸(GABA)的抗体的免疫染色。小白蛋白(PV)、钙视黄素(CR)、生长抑素(SOM)、钙结合蛋白(CB)和一氧化氮合酶(NOS)，以探索GABA能中间神经元的已知标记的共定位。结果表明，PV(100%)、SOM(89.8%)和CR(93.9%)染色的神经元多数为GABA阳性。PV免疫反应阳性神经元与CR、SOM和NOS表达细胞无重叠，与CB仅有少量重叠(5.3%)。PV免疫反应细胞占GABA能神经元的50.8%。第二类表达SOM的神经元占GABA能细胞的16.9%。这些细胞均不能同时定位于PV或CR，但1.7%的SOM神经元表达NOS，86.3%的SOM神经元表达CB免疫反应。第三类不同的CR表达细胞占GABA能神经元的17.0%。PV、CB、SOM、NOS均为阴性。CB表达的神经元是一个异质性的群体，包括GABA能和非GABA能细胞。我们的发现表明，大鼠17区的GABA能神经元至少有三个独立的家族，可以通过PV、CR和SOM的表达来识别。这些细胞占GABA能神经元的84.9%。这些结果扩展了以前在大鼠额叶无颗粒皮质中的观察结果，表明在视觉皮质中，抑制网络由相似的细胞类型组成。
In the cortex inhibition is mediated predominantly by GABAergic interneurons. Although all of these neurons use the same neurotransmitter, studies in the rat frontal cortex have shown that they are molecularly and physiologically diverse. It is not known whether similar subgroups of GABAergic neurons exist in primary visual cortex and how these different inhibitory neurons are inserted into specific cortical circuits. We have used immunostaining with antibodies against gamma aminobutyric acid (GABA). parvalbumin (PV), calretinin (CR), somatostatin (SOM), calbindin (CB) and nitric oxide synthase (NOS) to probe for colocalization of known markers of GABAergic interneurons. The results show that the majority of PV (100%), SOM (89.8%) and CR (93.9%) staining neurons are GABA positive. PV immunoreactive neurons constitute a distinct group that show no overlap with CR, SOM and NOS expressing cells and only a minor overlap (5.3%) with CB. PV immunoreactive cells account for 50.8% of GABAergic neurons. A second group of SOM expressing neurons accounts for 16.9% of GABAergic cells. None of these cells colocalize PV or CR, but 1.7% of SOM neurons stain for NOS and 86.3% show CB immunoreactivity. The third distinct group of CR expressing cells accounts for 17.0% of GABAergic neurons. All of these are PV, CB, SOM and NOS negative. CB expressing neurons represent a heterogeneous group that includes GABAergic and non GABAergic cells. Our findings indicate that GABAergic neurons in rat area 17 are organized in at least three separate families that can be identified by the expression of PV, CR and SOM. These cells account for 84.9% of GABAergic neurons. These results extend previous observations in rat frontal agranular cortex and suggest that in visual cortex the inhibitory network is composed of similar cell types.