Nucleotide Sequence of a cDNA Clone Encoding a Dehydrin-Like Protein from Stellaria longipes
Nucleotide Sequence of a cDNA Clone Encoding a Dehydrin-Like Protein from Stellaria longipes
复制标题
编码繁缕脱水蛋白样蛋白的 cDNA 克隆的核苷酸序列
作者：
Naima T. Wong;M. Zimmerman;E. Parker
许多不同基因的表达受阿坝和渗透胁迫的调节（Skriver和Mundy，1990）。这些基因和它们的产物已经在几种植物中被表征，并且它们包括大麦、玉米中的甜菜碱（Close等人，1989）和拟南芥（Rouse et al.，1992）;水稻中响应阿坝（RAB）的蛋白质（Mundy和Chua，1988; Yamaguchi-Shinozaki等，1989）;番茄中的TAS和le蛋白（Godoy等人，1990; Plant等人，1991）;棉花中的晚期胚胎发生丰富蛋白质（Baker等人，1988）;和烟草中的白藜芦醇苷（Singh等人，1987年）。这些蛋白质中的许多具有一定程度的结构相似性。长柄繁缕（Stellaria longipes Goldie）为双子叶多年生草本植物（Chinnappa and Morton，1984）。作为S. longipes complex，从Kananaskis Valley，阿尔伯塔，加拿大的天然群体构建叶cDNA文库。分离出一个随机cDNA克隆（H26）（表I），经测序，它类似于以前报道的来自大麦和A. thaliana（Close等人，1989; Rouse等人，1992）和来自水稻的RAB（Mundy和Chua，1988; YamaguchiShinozaki等人，1989年）。将其用于探测来自接受不同处理的植物的总叶RNA的北方印迹。杂交结果表明，H26的mRNA水平在叶片中诱导阿坝，PEG，或干旱处理，而茎似乎无反应。虽然整体结构与许多其他的类胡萝卜素非常相似，但S。longipes具有某些不同的结构特征（表I）。据我们所知，这是首次报道的繁缕属植物的DNA序列。
The expression of many different genes is regulated by ABA and osmotic stress (Skriver and Mundy, 1990). These genes and their products have been characterized in severa1 plants, and they include dehydrins in barley, corn (Close et al., 1989), and Arabidopsis thaliana (Rouse et al., 1992); proteins responsive to ABA (RAB) in rice (Mundy and Chua, 1988; Yamaguchi-Shinozaki et al., 1989); TAS and le proteins in tomato (Godoy et al., 1990; Plant et al., 1991); late embryogenesis-abundant proteins in cotton (Baker et al., 1988); and osmotin in tobacco (Singh et al., 1987). Many of these proteins share a certain degree of structural similarity. Stellaria longipes Goldie (Caryophyllaceae) is a dicotyledonous herbaceous perennial (Chinnappa and Morton, 1984). As part of a project on evolutionary studies of the S. longipes complex, a leaf cDNA library was constructed from a natural population in the Kananaskis Valley, Alberta, Canada. A random cDNA clone (H26) was isolated (Table I), which, on sequencing, resembled previously reported dehydrins from barley and A. thaliana (Close et al., 1989; Rouse et al., 1992) and RAB from rice (Mundy and Chua, 1988; YamaguchiShinozaki et al., 1989). It was used to probe northern blots of total leaf RNAs from plants receiving different treatments. The hybridization showed that the H26 mRNA levels in leaves were inducible by treatments of ABA, PEG, or drought, whereas stems seemed nonresponsive. Although the overall structure is very similar to many other dehydrins, the S. longipes dehydrin exhibits certain different structural features (Table I). To our knowledge, this is the first DNA sequence ever reported from the genus Stellaria.