POLYSPERMY-PREVENTING MECHANISMS IN GOLDEN-HAMSTER EGG
POLYSPERMY-PREVENTING MECHANISMS IN GOLDEN-HAMSTER EGG
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DOI：
10.1002/jez.1401800211
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发表时间：
1972-01-01
影响因子：
--
通讯作者：
YANAGIMACHI, R
中科院分区：
文献类型：
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作者：
BARROS, C;YANAGIMACHI, R
从人工授精的雌性受精卵中回收的受精卵的透明带对获能精子的反应与未受精卵的透明带不同。受精卵的卵带表现出一种对精子不敏感的反应，即“带状反应”，因此获得能力的精子既不能牢固地附着在它上面，也不能穿透它。另一方面，未受精卵的卵带没有表现出这种对精子的抵抗反应，许多精子附着并穿透它。卵带反应不仅见于新近穿透的卵子，而且贯穿整个卵裂。从最早观察到完全带状反应的受精阶段开始，估计这一反应在精子附着到卵黄后不到15分钟内完成。在人工受精5小时后从雌性受精卵中回收的受精卵中，100%在用胰酶去除透明带后被用于体外受精。以后回收卵子时，参考的发生率降低。在人工授精后9小时回收的卵子中，只有3%的卵子被引用。从卵子不再可参考的受精阶段来看，卵黄表面阻塞到多精受精的完成估计不少于两个小时，也不超过三个半小时。讨论了在体外受精卵中遇到多受精的可能原因。
The zona pellucida of a fertilized egg recovered from an artificially inseminated female reacts to capacitated spermatozoa differently from the zona pellucida of an unfertilized egg. The zona of a fertilized egg shows a sperm‐refractory response, “zona reaction,” so that capacitated spermatozoa neither firmly attach to nor penetrates it. On the other hand, the zona of an unfertilized egg shows no such sperm‐refractory response and many spermatozoa attach to and penetrate it. The zona reaction was observed not only in recently penetrated eggs but also throughout cleavage.Judging from the earliest fertilization stage at which we observed a complete zona reaction, it is estimated that this reaction is completed in less than 15 minutes after sperm attachment to the vitellus.One hundred per cent of the fertilized eggs recovered from females five hours after artificial insemination were refertilizedin vitroafter removal of the zona pellucida by trypsin‐treatment. The incidence of refertilization decreased when the eggs were recovered at later times. Only 3% of the eggs recovered at nine hours after artificial insemination were refertilized. Judging from the fertilization stage at which eggs were no longer refertilizable, it has been estimated that the completion of the vitelline surface block to polyspermy is established in not less than two nor more than three to three and one‐half hours.Possible causes of polyspermy encountered within vitrofertilized eggs are discussed.