Stem molecular signature of adipose-derived stromal cells
Stem molecular signature of adipose-derived stromal cells
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DOI：
10.1016/j.yexcr.2007.10.007
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发表时间：
2008-02-01
影响因子：
3.7
通讯作者：
Galie, Mirco
中科院分区：
文献类型：
--
作者：
Peroni, Daniele;Scambi, Ilaria;Galie, Mirco
最近的研究表明，脂肪组织是一种丰富且容易获得的基质祖细胞（ADSCs，脂肪来源的基质细胞）的来源，类似于从成人骨髓中获得的间充质干细胞（MSCs）。然而，这两种谱系的鉴定仍然存在争议，甚至ADSCs的干细胞性质也受到质疑。在这项研究中，我们研究了ADSCs和BM-MSCs的“干性转录谱”，有两个目的：(1)比较它们的“干细胞分子特征”；(2)剖析它们参与组织发育、体内平衡和修复的分子的组成表达模式。除了参与基质重塑和成人组织血管生成和修复的几个分子外，我们还检测到基因UTF-1、Nodal和Snail2的表达，这些基因已知是由。胚胎干细胞，但从未在其他干细胞谱系中描述过。此外，我们首次描述了人BM-MSCs和ADSCs的CD44剪接变体的转录谱，这些剪接变体在胚胎发育期间的细胞运输、成人组织稳态和肿瘤传播中起决定作用。因此，我们的研究结果有力地支持了ADSCs和BM-MSCs之间的密切关系，表明MSCs和胚胎干细胞之间存在意想不到的相似性，并可能支持ADSCs的潜在治疗应用。(c) 2007爱思唯尔公司版权所有。
It has recently been shown that adipose tissue is an abundant and easily accessible source of stromal progenitor cells (ADSCs, adipose-derived stromal cells), resembling the mesenchymal stem cells (MSCs) obtained from adult bone marrow. However, the identification of these two lineages is still controversial and even the stem cell nature of ADSCs is doubted. In this study, we examined the "stemness transcriptional profile of ADSCs and BM-MSCs, with two aims: (1) to compare their "stem cell molecular signature" and (2) to dissect their constitutive expression pattern for molecules involved in tissue development, homeostasis and repair. As well as several molecules involved in matrix remodeling and adult tissue angiogenesis and repair, we detected, the expression of genes UTF-1, Nodal, and Snail2, which are known to be expressed by. embryonic stem cells but have been never described in other stem lineages. In addition, for the first time we described the transcriptional profile of human BM-MSCs and ADSCs for the CD44 splice variants, which are determinant in cell trafficking during embryonic development, in adult tissue homeostasis and also in tumor dissemination.Thus, our findings strongly support a close relationship between ADSCs and BM-MSCs, suggest an unexpected similarity between MSCs and embryonic stem cells, and possibly support the potential therapeutic application of ADSCs. (c) 2007 Elsevier Inc. All rights reserved.