Generation of Measles Virus with a Segmented RNA Genome
Generation of Measles Virus with a Segmented RNA Genome
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DOI：
10.1128/jvi.80.9.4242-4248.2006
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发表时间：
2006-05
影响因子：
5.4
通讯作者：
M. Takeda;Y. Nakatsu;S. Ohno;Fumio Seki;Maino Tahara;T. Hashiguchi;Y. Yanagi
中科院分区：
文献类型：
--
作者：
M. Takeda;Y. Nakatsu;S. Ohno;Fumio Seki;Maino Tahara;T. Hashiguchi;Y. Yanagi
摘要 分类为单链病毒目的病毒具有单个不分段的 RNA 分子作为基因组，并采用类似的基因组复制和基因表达策略。麻疹病毒 (MeV) 是单负病毒目副粘病毒科的成员，具有两个或三个 RNA 基因组片段（2 seg-或 3 seg-MeV），其感染性颗粒是使用高效反向遗传学系统生成的。病毒的所有 RNA 片段都被设计为具有真正的 3' 和 5' 自我互补末端，类似于本质上具有多个 RNA 基因组片段的负链 RNA 病毒。 2 seg-和3 seg-MeV 在培养细胞中是可行的并且复制良好。 3 seg-MeV 可以容纳多达六个额外的转录单位，其中五个被证明能够有效表达外源蛋白质。这些数据表明 MeV 基因组可以被分段，从而为负链 RNA 病毒与不分段或分段 RNA 基因组的差异提供了实验见解。他们还阐述了开发包含多个额外转录单元的单负病毒衍生载体的新策略。
ABSTRACT Viruses classified in the order Mononegavirales have a single nonsegmented RNA molecule as the genome and employ similar strategies for genome replication and gene expression. Infectious particles of Measles virus (MeV), a member of the family Paramyxoviridae in the order Mononegavirales, with two or three RNA genome segments (2 seg- or 3 seg-MeV) were generated using a highly efficient reverse genetics system. All RNA segments of the viruses were designed to have authentic 3′ and 5′ self-complementary termini, similar to those of negative-stranded RNA viruses that intrinsically have multiple RNA genome segments. The 2 seg- and 3 seg-MeV were viable and replicated well in cultured cells. 3 seg-MeV could accommodate up to six additional transcriptional units, five of which were shown to be capable of expressing foreign proteins efficiently. These data indicate that the MeV genome can be segmented, providing an experimental insight into the divergence of the negative-stranded RNA viruses with nonsegmented or segmented RNA genomes. They also illustrate a new strategy to develop mononegavirus-derived vectors harboring multiple additional transcriptional units.