Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro.
Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro.
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DOI：
10.2741/1835
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Y. Q. Yang;X. Li;A. Rabie;M. Fu;D. Zhang
中科院分区：
文献类型：
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作者：
Y. Q. Yang;X. Li;A. Rabie;M. Fu;D. Zhang
未标记的机械应变作用于骨骼会导致骨骼重塑。在口腔中，还不清楚这样的机械力是如何通过正畸方式移动牙齿来诱导牙槽骨重塑的。众所周知，破骨细胞是唯一负责骨吸收的细胞，而破骨细胞的形成和活性受成骨细胞的调节。因此，人们认为成骨细胞不仅在骨形成中发挥重要作用，而且在骨重建中也起着重要作用。因此，本研究的目的是检测机械力对人牙周膜细胞的影响，以及体外培养的人牙周膜细胞是否具有成骨特性。方法体外培养人视网膜色素上皮细胞，间歇性拉伸24小时。分别于0、2、4、6、12、24 h检测碱性磷酸酶(ALP)、骨钙素(OCN)和护骨素(OPG)在mRNA和蛋白水平的表达。结果在无刺激条件下，原位杂交检测到人PDL细胞ALP和OPG基因的表达，但未检测到OCN基因的表达。机械力作用24小时内，ALP基因表达上调，OPG基因表达下调。在24小时负荷周期的后期，机械力诱导OCN基因的表达。分泌蛋白的变化与在mRNA水平上看到的结果相似。结论人牙周膜细胞在间歇力作用下表达成骨细胞表型，在牙槽骨改建中起重要作用。
UNLABELLED Mechanical strain applied to bone leads to bone remodeling. In the oral cavity, it is unclear how such mechanical force applied to move teeth orthodontically induces alveolar bone remodeling. It is known that osteoclasts are the only cells that are responsible for bone resorption, while the formation and activity of osteoclasts are regulated by osteoblasts. So it is believed that osteoblasts play an important role not only in bone formation but in bone remodeling as well. Therefore, the purpose of this study was to examine the effect of mechanical force on human periodontal ligament (PDL) cells and whether they express osteoblastic characters in vitro. METHODS Human PDL cells cultured in vitro were loaded with intermittently stretching force for 24 hours. The expression of alkaline phosphatase (ALP), osteocalcin (OCN) and osteoprotegerin (OPG) were detected at mRNA and protein levels at 0, 2nd, 4th, 6th, 12th, 24th hours after intermittent force loading. RESULTS Without any stimulation, ALP and OPG mRNA expressions were detected in human PDL cells by in-situ hybridization, but not that of OCN mRNA. ALP mRNA signal was up-regulated and that of OPG was down-regulated by mechanical force within 24 hours. OCN mRNA expression was induced by mechanical force in the late phase of the 24-hours loading cycle. The changes in secreted proteins showed similar results with those seen at the mRNA level. CONCLUSION Human PDL cells express osteoblastic phenotypes under intermittent force loading and play a role in alveolar bone remodeling.