Nanozyme-Catalyzed Cascade Reactions for Mitochondria-Mimicking Oxidative Phosphorylation
Nanozyme-Catalyzed Cascade Reactions for Mitochondria-Mimicking Oxidative Phosphorylation
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纳米酶催化的模拟线粒体氧化磷酸化的级联反应
DOI：
10.1002/anie.201813771
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Li Junbai
中科院分区：
文献类型：
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作者：
Xu Youqian;Fei Jinbo;Li Guangle;Yuan Tingting;Xu Xia;Li Junbai
产生生物能的多酶级联反应是自然氧化磷酸化所必需的。然而，体外应用受到催化活性对环境适应的敏感性的阻碍。在此，我们探索了纳米酶催化的级联反应，在组装的混合结构中模拟线粒体的氧化磷酸化。合成了含有金纳米颗粒的中空二氧化硅微球，以促进两种酶样催化反应，在氧气存在下将葡萄糖转化为葡萄糖酸。由此产生的跨膜质子梯度驱动天然ATP合成酶在表面重组，将ADP和无机磷酸盐转化为ATP。组装的结构具有高氧化磷酸化活性，可与天然线粒体相媲美。这项研究为探索生物能源供应系统提供了一种新的自然-人工混合原型，并为ATP驱动的生物应用提供了巨大的希望。
Multiple‐enzyme‐involving cascade reactions that yield bioenergy are necessary in natural oxidative phosphorylation. However, in vitro applications are hampered by the sensitivity of catalytic activity to environmental adaptation. Herein, we explore nanozyme‐catalyzed cascade reactions in an assembled hybrid architecture for mitochondria‐mimicking oxidative phosphorylation. Hollow silica microspheres containing trapped gold nanoparticles were synthesized to promote two enzyme‐like catalytic reactions that transform glucose into gluconic acid in the presence of oxygen. The resulting transmembrane proton gradient drives natural ATP synthase reconstituted on the surface to convert ADP and inorganic phosphate into ATP. The assembled architecture possesses high activity for oxidative phosphorylation, comparable to that of natural mitochondria. This study provides a new natural–artificial hybrid prototype for exploring bioenergy supply systems and holds great promise for ATP‐powered bioapplications.