Effect of protamine on the permeability and structure of rat peritoneum.
Effect of protamine on the permeability and structure of rat peritoneum.
复制标题
鱼精蛋白对大鼠腹膜通透性和结构的影响。
作者：
N. Alavi;E. Lianos;G. Andres;C. Bentzel
在大鼠腹膜透析模型中，研究了多阳离子蛋白鱼精蛋白对间皮通透性和超微结构的影响。腹腔注射不同浓度的硫酸鱼精蛋白后，测定大鼠腹腔对尿素和菊粉的通透性。与网膜超微结构有功能相关性。鱼精蛋白浓度在5 ~ 30微克/毫升之间降低了对菊粉的渗透性，但对尿素的渗透性没有明显改变。在浓度为30 ~ 75微克/毫升时，鱼精蛋白对尿素的渗透性提高50%，对菊粉的渗透性提高20%。间皮细胞显示微绒毛缺失，亚膜质、细胞质微丝轻微解体，膜内闭塞连接结构无明显变化。效果部分可逆。当浓度为100微克/毫升时，菊粉的渗透性不可逆地增加了一倍，但对尿素的渗透性没有类似的影响，这与焦区闭塞连接的破坏有关。这种对鱼精蛋白的复杂反应表明至少有两种经腹腔扩散途径，跨细胞和细胞旁扩散途径。间皮细胞闭塞连接可能是后一途径的扩散屏障。
The effect of protamine, a polycationic protein, on the mesothelial permeability and ultrastructure was evaluated in a rat model of peritoneal dialysis. The peritoneal permeability to urea and inulin were measured after the intraperitoneal instillation of protamine sulfate in varying concentrations. A functional correlation was made with the ultrastructure of omentum. Protamine concentrations between 5 and 30 micrograms/ml decreased the permeability to inulin without significantly altering that of urea. At concentrations from 30 to 75 micrograms/ml, protamine increased permeability to urea by 50% and to inulin by 20%. Mesothelial cells revealed a loss of microvilli and minor degrees of disorganization of submembranous, cytoplasmic microfilaments without significant changes in the intramembranous structure of occluding junctions. Effects were partially reversible. At a concentration of 100 micrograms/ml, there was an irreversible doubling in permeability to inulin without a comparable effect on permeability to urea associated with the disruption of occluding junctions in focal areas. This complex response to protamine suggests at least two transperitoneal diffusion pathways, transcellular and paracellular. The mesothelial cell occluding junctions may be a diffusion barrier in the latter pathway.