U14 BASE-PAIRS WITH 18S RIBOSOMAL-RNA - A NOVEL SNORNA INTERACTION REQUIRED FOR RIBOSOMAL-RNA PROCESSING
U14 BASE-PAIRS WITH 18S RIBOSOMAL-RNA - A NOVEL SNORNA INTERACTION REQUIRED FOR RIBOSOMAL-RNA PROCESSING
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DOI：
10.1101/gad.9.19.2433
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发表时间：
1995-10-01
影响因子：
10.5
通讯作者：
FOURNIER, MJ
中科院分区：
文献类型：
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作者：
LIANG, WQ;FOURNIER, MJ
U14是一种保守的小核仁RNA（snoRNA），是酵母18 S rRNA加工所必需的。与18 S rRNA具有强互补性的两个长序列（13和14个核苷酸）的存在表明U14与pre-rRNA碱基配对。直接结合的证据是通过显示这些U14元件中的突变模拟U14缺失，并且可以通过18 S RNA中的补偿性序列变化来拯救功能而开发的。U14元件在功能上是相互依赖的，表明两者都参与结合。折叠模型预测结合可能同时通过两个rRNA元件发生。U14在rRNA折叠，成熟和核糖体组装中的潜在作用进行了讨论。U14是与rRNA具有长互补性的几种snoRNA之一，也是这类snoRNA中第一个与rRNA直接相互作用的snoRNA。
U14 is a conserved small nucleolar RNA (snoRNA) required for processing of yeast 18S rRNA. The presence of two long sequences (13 and 14 nucleotides) with strong complementarity to 18S rRNA suggests that U14 base-pairs with pre-rRNA. Evidence of direct binding was developed by showing that mutations in these U14 elements mimic U14 depletion and that function can be rescued by a compensatory sequence change in 18S RNA. The U14 elements are functionally interdependent, indicating that both participate in binding. folding models predict that binding might occur through both rRNA elements simultaneously. Potential roles of U14 in rRNA folding, maturation, and ribosome assembly are discussed. U14 is one of several snoRNAs with long complementarities to rRNA and the first snoRNA in this class shown to interact directly with rRNA.