Genetically variant populations of Paragonimus proliferus Hsia & Chen, 1964 from central Vietnam
Genetically variant populations of Paragonimus proliferus Hsia & Chen, 1964 from central Vietnam
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DOI：
10.1017/s0022149x12000090
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发表时间：
2013-06-01
影响因子：
1.6
通讯作者：
Nawa, Y.
中科院分区：
文献类型：
--
作者：
Doanh, P. N.;Hien, H. V.;Nawa, Y.
在大约50种名义上的并殖吸虫中，增殖并殖吸虫是一种相当罕见的种，仅在中国云南省发现，直到最近我们在越南北部靠近中国云南的赖州省发现了该种。这里我们添加广平省，越南中部作为一个新的流行区P. proliferus。在山地蟹（Potamiscus tannanti）中发现的大型囊性囊蚴在形态学上被鉴定为P. proliferus，并通过分子分析进一步证实了这一点。广平省增殖p.p proliferus居群的第2个内部转录间隔序列（ITS2）与越南北部赖州省和中国云南省的增殖p.p proliferus居群完全（100%）相同。然而，广平人群的细胞色素氧化酶亚基1 （CO1）基因序列与先前报道的越南北部和中国云南人群差异显著（5.6%）。系统进化树分析结果显示，广平居群CO1序列与越南北部和中国云南居群聚集在一起，自举值为75%。这是第一次记录到增殖质p.p proliferus的遗传变异种群，其分布在地理上远离先前报道的越南北部和中国云南交界的流行区，这表明增殖质p.p proliferus在印度支那半岛（或东南亚）的分布可能比预期的要广泛得多。
Among about 50 nominal Paragonimus species, Paragonimus proliferus is rather a rare species, found only in Yunnan province, China, until our recent discovery of this species in Lai Chau province, northern Vietnam close to Yunnan, China. Here we add Quang Binh province, central Vietnam as a new endemic area of P. proliferus. Large excysted metacercariae found in mountainous crabs, Potamiscus tannanti, were morphologically identified as P. proliferus, which was confirmed further by molecular analyses. Second internal transcribed spacer (ITS2) sequences of the P. proliferus population in Quang Binh province were completely (100%) identical with those of P. proliferus populations in Lai Chau province, northern Vietnam and Yunnan province, China. However, cytochrome oxidase subunit 1 (CO1) gene sequences of Quang Binh population were significantly different (5.6%) from that of previously reported northern Vietnam and Yunnan, China populations. A phylogenetic tree revealed that all CO1 sequences of P. proliferus Quang Binh population formed a distinct group, which was clustered with northern Vietnam and Yunnan, China populations with the bootstrap value of 75%. This is the first record of the genetically variant population of P. proliferus, distribution of which is geographically remote from the previously reported endemic areas in the border between northern Vietnam and Yunnan, China, suggesting that P. proliferus may be much more widely distributed in the Indochina peninsula (or South-East Asia) than expected.