Detection and typing by molecular techniques of respiratory viruses in children hospitalized for acute respiratory infection in Rome, Italy
Detection and typing by molecular techniques of respiratory viruses in children hospitalized for acute respiratory infection in Rome, Italy
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DOI：
10.1002/jmv.20832
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发表时间：
2007-04-01
影响因子：
12.7
通讯作者：
Antonelli, Guido
中科院分区：
文献类型：
--
作者：
Pierangeli, Alessandra;Gentile, Massimo;Antonelli, Guido
由于涉及的病原体数量庞大且不断增加，目前尚未对大量呼吸道病毒进行检测来诊断急性呼吸道感染。对罗马儿科连续因急性呼吸道感染住院的儿童进行了一项为期一年的研究，以鉴定相关病毒的特征。 227 名被诊断患有哮喘、细支气管炎、支气管肺炎或喉气管支气管炎的儿童参加了这项研究。采用分子方法，使用特异性逆转录（RT）-PCR检测检测13种呼吸道病毒，包括偏肺病毒（hMPV）和新型冠状病毒NL63和HKU1；对大多数扩增片段进行测序以确认阳性结果并区分菌株。 97份样本（42.7%）检出病毒病原体，其中双重感染率为4.8%； 17.2%的儿童检测到呼吸道合胞病毒（RSV），其次是鼻病毒（9.7%）、副流感病毒3型（PIV3）（7.5%）和甲型流感（4.4%）。有趣的是，超过一半以鼻病毒为唯一呼吸道病原体的患者（9/17）患有肺炎。 HMPV 在 3 月和 6 月出现两个高峰，感染 3 岁以下儿童，引起细支气管炎和肺炎。描述了 1 例 NL63 感染病例，记录了 NL63 在意大利中部的传播情况。总之，建议使用全面的基于 PCR 的检测来确定呼吸道感染患者的病毒病原体负担。
Detection of a broad number of respiratory viruses is not undertaken currently for the diagnosis of acute respiratory infection due to the large and always increasing list of pathogens involved. A 1-year study was undertaken on children hospitalized consecutively for acute respiratory infection in a Pediatric Department in Rome to characterize the viruses involved. Two hundred twenty-seven children were enrolled in the study with a diagnosis of asthma, bronchiolitis, bronchopneumonia, or laringo-tracheo bronchitis. A molecular approach was adopted using specific reverse transcription (RT)-PCR assays detecting 13 respiratory viruses including metapneumovirus (hMPV) and the novel coronaviruses NL63 and HKU1; most amplified fragments were sequenced to confirm positive results and differentiate the strain. Viral pathogens were detected in 97 samples (42.7%), with 4.8% of dual infections identified; respiratory syncytial virus (RSV) was detected in 17.2% of children, followed by rhinovirus (9.7%), parainfluenza virus type 3 (PIV3) (7.5%), and influenza type A (4.4%). Interestingly, more than half the patients (9/17) that have rhinovirus as the sole respiratory pathogen had pneumonia. HMPV infected children below 3 years in two peaks in March and June causing bronchiolitis and pneumonia. One case of NL63 infection is described, documenting NL63 circulation in central Italy. In conclusion, the use of a comprehensive number of PCR-based tests is recommended to define the burden of viral pathogens in patients with respiratory tract infection.