Efficient Conversion of Fructose-Based Biomass into Lipids with Trichosporon fermentans Under Phosphate-Limited Conditions
Efficient Conversion of Fructose-Based Biomass into Lipids with Trichosporon fermentans Under Phosphate-Limited Conditions
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DOI：
10.1007/s12010-017-2536-y
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发表时间：
2018-01-01
影响因子：
3
通讯作者：
Lin, Xinping
中科院分区：
文献类型：
--
作者：
Bao, Ruiqi;Wu, Xiangying;Lin, Xinping
限制氮供应已被常规地用作指导脂质生物合成的主调节剂。然而，这种策略不适用于富含氮的底物，如耶路撒冷（JA），一种基于果糖的生物质，同时难以获得高的碳氮（C/N）摩尔比。在这项研究中，一种替代的策略，以促进油脂酵母Trichosporon fermentans CICC 1368的脂质积累的限制磷供应，并实施了这种策略与JA水解产物作为底物。我们发现，脂质积累与T.发酵剂。在初始C/P比为6342的培养基中，分析了细胞生长和脂质产生的时间过程，细胞脂质含量可达干生物量的48.5%。此外，JA水解物作为微生物油脂积累的底物，在高C/P摩尔比条件下，油脂产量、油脂含量和油脂系数分别提高了10%、30%和34%。结果表明，限磷能有效地提高糖向脂类的转化率。限制磷为用富氮天然材料生产微生物脂质的问题提供了一个有前途的解决方案。
Limiting nitrogen supply has been routinely used as the master regulator to direct lipid biosynthesis. However, this strategy does not work with nitrogen-rich substrates, such as Jerusalem artichoke (JA), a fructose-based biomass, while it is difficult to obtain a high carbon-to-nitrogen (C/N) molar ratio. In this study, an alternative strategy to promote lipid accumulation by the oleaginous yeast Trichosporon fermentans CICC 1368 was developed by limiting phosphorous supply, and this strategy was implemented with JA hydrolysate as substrate. We showed that lipid accumulation was directly correlated with the C/P ratio of the culture media for T. fermentans. The time course of cell growth and lipid production was analyzed in a media with an initial C/P ratio of 6342, and the cellular lipid content could reach up to 48.5% of dry biomass. Moreover, JA hydrolysates were used as substrate for microbial lipid accumulation, under high C/P molar ratio condition, lipid yield, lipid content, and lipid coefficient increased by 10, 30, and 34%, respectively. It showed that by limiting phosphorus, the conversion of sugar into lipids can be improved effectively. Limiting phosphorus provides a promising solution to the problem of microbial lipid production with nitrogen-rich natural materials.