Micromethod for the determination of bupivacaine in maternal and foetal blood during obstetric analgesia
Micromethod for the determination of bupivacaine in maternal and foetal blood during obstetric analgesia
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产科镇痛时母胎血中布比卡因的微量测定
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
P. Belfrage
中科院分区：
文献类型：
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作者：
A. Berlin;B. Persson;P. Belfrage
本文介绍了一种测定分娩镇痛时母血和胎儿血中布比卡因浓度的方法。首先将药物和内标物1-戊基-2-（2，6-二甲苯基氨基甲酰基）-哌啶萃取至二氯甲烷中。加入高氯酸以将布比卡因和标准品作为高氯酸盐保留在有机相中，实现与疏水性较低的胺的选择性分离。然后将布比卡因和标准品重新萃取到硫酸中，然后用二氯甲烷纯化。最后将水提取物碱化，并将化合物提取至10 μl二氯甲烷中。使用3% OV-17色谱柱通过气相色谱法分析该浸提液。该方法在治疗浓度下的标准偏差约为10%，可以合理准确地测定的最低水平为15 ng ml-1。
A method for the determination of bupivacaine in maternal and foetal blood during obstetric analgesia is described. The drug and the internal standard, 1‐pentyl‐2‐(2,6‐xylylcarbamoyl)‐piperidine, are initially extracted into methylene chloride. Perchloric acid is added to retain bupivacaine and the standard as Perchlorates in the organic phase accomplishing a selective separation from less hydrophobic amines. Bupivacaine and the standard are then re‐extracted into sulphuric acid, followed by a purification with methylene chloride. The aqueous extract is finally made alkaline and the compounds extracted into 10 μl methylene chloride. This extract is analysed by gas chromatography using a 3% OV‐17 column. The standard deviation of the method at therapeutic concentrations is about 10% and the lowest level which can be determined with reasonable accuracy is 15 ng ml−1.