De novo assembled transcriptome of horned gall aphid, Schlechtendalia chinensis Bell, suggest changes in functional gene expression during host alternation
De novo assembled transcriptome of horned gall aphid, Schlechtendalia chinensis Bell, suggest changes in functional gene expression during host alternation
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从头组装的角瘿蚜 (Schlechtendalia chinensis Bell) 转录组表明宿主交替期间功能基因表达的变化
DOI：
10.1111/1748-5967.12179
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发表时间：
2016
影响因子：
1.3
通讯作者：
Zhang Chuan-Xi
中科院分区：
文献类型：
--
作者：
Wang Si-Liang;Yang Zi-Xiang;Yang Pu;Zhang Chuan-Xi
角倍蚜是我国最有经济价值的刺蚜。它约占中国五倍子产量的75%，这是重要的医疗和化学用途。角瘿蚜在夏季以盐肤木为食，诱发虫瘿，在苔藓类走灯藓上越冬。然而，中华小蠊的序列信息有限，寄主更替过程中功能基因的表达变化仍不清楚。在这项研究中，使用高通量测序技术对角倍蚜的转录组进行测序。获得了大约4900万个读段，并组装成99366个转录本和47400个单基因。使用BLAST检索对主要数据库（如NCBI nr和Swiss-Prot）进行注释，并通过GO、KOG和KEGG进行富集分析。对春、秋迁蚜差异表达基因进行比较，发现许多与解毒、消化等功能基因相关的基因表达水平发生了变化，反映了角倍蚜对不同季节和寄主的适应能力。蜡生物合成的功能基因在春迁飞蚜虫中上调。本研究分析了春、秋移蚜在寄主转换过程中基因表达谱的差异，以探讨寄主转换过程中真实的功能基因的变化。转录组的研究为了解瘿蚜寄主转换过程中的功能基因及其相关的生理和分子生物学特性提供了基础。
The horned gall aphid, Schlechtendalia chinensis Bell, is the most economically valuable galling aphid. It accounts for approximately 75% of Chinese gallnut production, which is important for medical and chemical purposes. The horned gall aphid feeds on the Chinese sumac tree, Rhus chinensis, during the summer, induces galls, and overwinters on the moss Plagiomnium spp. However, the sequence information of S. chinensis is limited, and the functional gene expression changes during host alternation remain obscure. In this study, the transcriptome of the horned gall aphid was sequenced using high‐throughput sequencing technology. Approximately 49 million reads were obtained and were assembled into 99 366 transcripts and 47 400 unigenes. Annotation was performed using a BLAST search against primary databases such as NCBI nr and Swiss‐Prot, as well as enrichment analysis by GO, KOG and KEGG. Differentially expressed genes between spring and autumn migrant aphids were compared, and the expression levels of many genes related to detoxification, digestion and other functional genes were found to have changed, reflecting the adaptation ability of horned gall aphids in different seasons and hosts. Functional genes in wax biosynthesis were upregulated in spring migrant aphids. This study analyzed the expression profile difference between spring and autumn migrant aphids to approach the real functional genes change during host alternation. The transcriptome provides a basis to understanding the functional genes during gall aphid host alternation and the related physiological and molecular characteristics.