Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, from the Venom of the Spider Heteropoda venatoria.
Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, from the Venom of the Spider Heteropoda venatoria.
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一种新型杀虫毒素 mu-sparatoxin-Hv2（来自蜘蛛 Heteropoda venatoria 毒液）的纯化和表征。
DOI：
10.3390/toxins10060233
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发表时间：
2018-06-07
期刊：
影响因子：
4.2
通讯作者：
Liu Z
中科院分区：
文献类型：
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作者：
Xiao Z;Zhang Y;Zeng J;Liang S;Tang C;Liu Z
蜘蛛毒液对蟑螂有致死作用，可作为天然杀虫剂的来源。本研究鉴定了一种新的蟑螂电压门控钠通道(NAWS)拮抗剂μ-SPRTX-Hv2(简称μ-SPRTX-Hv2)。μ-SPRTX-Hv2由37个氨基酸组成，含有6个保守的半胱氨酸。我们采用化学合成法合成了该毒素。该毒素对蟑螂有致死作用，LD50值为2.8nmol/g体重。电生理实验结果表明，该毒素能有效地阻断蟑螂背侧不成对正中神经(DUM)神经元的NAV，IC_(50)为833.7±132.2 nM，但对DUM电压门控钾通道(KVS)和高压激活钙通道(HVA CAVs)无明显影响。该毒素也不影响大鼠背根神经节(DRG)神经元的NAV、HVA Cavs和KVS，以及NAV亚型NaV1.3-1.5、Nav1.7和NaV1.8。μ-SPRTX-Hv2以7.0μg/g剂量给小鼠腹腔注射，未观察到毒液中毒症状。综上所述，μ-SPRTX-Hv2是一种从蛇毒中分离出来的新杀虫毒素。它可能通过阻断昆虫的导航而发挥其作用，是开发生物杀虫剂的候选药物。
The venom of the spider Heteropoda venatoria produced lethal effect to cockroaches as reported in our previous study, and could be a resource for naturally-occurring insecticides. The present study characterized a novel cockroach voltage-gated sodium channels (NaVs) antagonist, μ-sparatoxin-Hv2 (μ-SPRTX-Hv2 for short), from this venom. μ-SPRTX-Hv2 is composed of 37 amino acids and contains six conserved cysteines. We synthesized the toxin by using the chemical synthesis method. The toxin was lethal to cockroaches when intraperitoneally injected, with a LD50 value of 2.8 nmol/g of body weight. Electrophysiological data showed that the toxin potently blocked NaVs in cockroach dorsal unpaired median (DUM) neurons, with an IC50 of 833.7 ± 132.2 nM, but it hardly affected the DUM voltage-gated potassium channels (KVs) and the DUM high-voltage-activated calcium channels (HVA CaVs). The toxin also did not affect NaVs, HVA CaVs, and Kvs in rat dorsal root ganglion (DRG) neurons, as well as NaV subtypes NaV1.3–1.5, NaV1.7, and NaV1.8. No envenomation symptoms were observed when μ-SPRTX-Hv2 was intraperitoneally injected into mouse at the dose of 7.0 μg/g. In summary, μ-SPRTX-Hv2 is a novel insecticidal toxin from H. venatoria venom. It might exhibit its effect by blocking the insect NaVs and is a candidate for developing bioinsecticide.