Optimizing Drosophila olfactory learning with a semi-automated training device.
Optimizing Drosophila olfactory learning with a semi-automated training device.
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使用半自动训练设备优化果蝇的嗅觉学习。
DOI：
10.1016/j.jneumeth.2010.02.007
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发表时间：
2010
影响因子：
3
通讯作者：
Tabata,Tetsuya
中科院分区：
文献类型：
--
作者：
Murakami,Satoshi;Dan,Chuntao;Zagaeski,Brendan;Maeyama,Yuko;Kunes,Sam;Tabata,Tetsuya
果蝇的嗅觉厌恶条件反射已经成为一个强有力的模型系统，用来阐明记忆形成的分子和神经机制。在典型的方案中，苍蝇暴露于恒定的气味流，同时在手动设备的调节管中接收脉冲电击。我们设计了一种简单、低成本的半自动调节装置，通过计算控制气味和震动的传递。采用基于生物传感器的气味传感器来监测训练管中气味浓度的变化。因此，该系统允许电击与训练管中的气味浓度精确匹配。我们发现，短期记忆的表现得到改善与脉冲气味流协议，其中气味是在短脉冲，每个配对电击，而不是作为一个恒定的流量。脉冲气味流的效果可能归因于“条件接近”现象，即在气味脉冲结束前出现电击时，会诱导朝向气味的接近。我们的数据表明，该系统是适用于研究嗅觉记忆的形成和检查的条件反射参数的详细程度不实用的手动装置。
Drosophila olfactory aversive conditioning has served as a powerful model system with which to elucidate the molecular and neuronal mechanisms underlying memory formation. In the typical protocol, flies are exposed to a constant odor stream while receiving a pulsed electric shock in the conditioning tube of a manual apparatus. We have devised a simple, low-cost semi-automated conditioning apparatus that computationally controls the delivery of odor and shock. A semiconductor-based odor sensor is employed to monitor the change of odor concentration in the training tube. The system thus allows electric shocks to be precisely matched with odor concentration in the training tube. We found that short-term memory performance was improved with a pulsed odor flow protocol, in which odor is presented in short pulses, each paired with electric shock, rather than as a constant flow. The effect of pulsed odor flow might be ascribed to the phenomenon of ‘conditioned approach’, where approach toward an odor is induced when the electric shock is presented before odor pulse ends. Our data shows that the system is applicable to the study of olfactory memory formation and to the examination of conditioning parameters at a level of detail not practical with a manual apparatus.