Dietary Apigenin Relieves Body Weight and Glycolipid Metabolic Disturbance via Pro-Browning of White Adipose Mediated by Autophagy Inhibition.
Dietary Apigenin Relieves Body Weight and Glycolipid Metabolic Disturbance via Pro-Browning of White Adipose Mediated by Autophagy Inhibition.
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DOI：
10.1002/mnfr.202200763
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发表时间：
2023-07
影响因子：
5.2
通讯作者：
Shaofeng Xiong;Shumin Yu;Kun Wang;Xiaowei Xiong;Min Xia;Guohua Zeng;Qiren Huang
中科院分区：
文献类型：
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作者：
Shaofeng Xiong;Shumin Yu;Kun Wang;Xiaowei Xiong;Min Xia;Guohua Zeng;Qiren Huang
芹菜素（Apigenin，AP）具有抗炎、降血脂等多种药理活性，研究表明AP在体外可降低脂肪细胞脂质蓄积。然而，目前还不清楚AP是否以及如何促进脂肪褐变。因此，本研究采用小鼠肥胖模型和体外前脂肪细胞诱导模型，探讨了AP对糖脂代谢、布朗宁和自噬的影响及其可能的机制。方法与结果肥胖小鼠灌胃AP（0.1mg·g ~（-1）·d ~（-1））4 wk，同时分别用指定浓度的AP处理分化中的前脂肪细胞48 h。代谢表型，脂质积累，脂肪褐变分别进行了评估的形态，功能和具体的标记分析。结果表明，AP能减轻肥胖小鼠体重，改善糖脂代谢紊乱，降低胰岛素抵抗，这与AP在体内和体外的促褐化作用有关。此外，研究发现AP的促褐化作用是通过激活PI 3 K-Akt-mTOR通路介导的自噬抑制来实现的。结论：该发现强调了自噬抑制促进白色脂肪细胞的布朗宁，并表明AP可以预防和治疗肥胖及其相关的代谢紊乱。
SCOPE Apigenin (AP) has many pharmacological activities, including anti-inflammation, hyperlipidemia-lowering, and so on. Previous studies show that AP can reduce lipid accumulation in adipocytes in vitro. However, it remains unclear whether and how AP can promote fat-browning. Therefore, mouse obesity model and preadipocyte induction model in vitro are used to investigate the effects of AP on glycolipid metabolism, browning and autophagy as well as the possible mechanisms. METHODS AND RESULTS The obese mice are intragastrically administrated with AP (0.1 mg g-1 d-1 ) for 4 weeks; meanwhile, the differentiating preadipocytes are respectively treated with the indicated concentrations of AP for 48 h. Metabolic phenotype, lipid accumulation, and fat-browning are respectively evaluated by morphological, functional, and specific markers analysis. The results show that AP treatment alleviates the body weight, glycolipid metabolic disorder, and insulin resistance in the obese mice , which is contributed to the pro-browning effects of AP in vivo and in vitro. Moreover, the study finds that the pro-browning effect of AP is accomplished through autophagy inhibition mediated by the activation of PI3K-Akt-mTOR pathway. CONCLUSIONS The findings highlight that autophagy inhibition promotes the browning of white adipocytes and suggest that AP would prevent and treat obesity and the associated metabolic disorders.