Timing and rate of genome variation in triticale following allopolyploidization
Timing and rate of genome variation in triticale following allopolyploidization
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DOI：
10.1139/g06-078
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发表时间：
2006-08-01
期刊：
影响因子：
3.1
通讯作者：
Gustafson, J. Perry
中科院分区：
文献类型：
--
作者：
Ma, Xue-Feng;Gustafson, J. Perry
研究了小麦-黑麦属间异源多倍化诱导基因组变异的时间和速率。- 黑麦（Secale cereale L.）利用EcoRI-MseI（E-M）和PstI-MseI（P-M）两对引物对杂种小黑麦（x Triticosecale Wittmack）进行了AFLP分析。结果表明，异源多倍化诱导了小黑麦基因组序列变异，大部分变异发生在远缘杂交之后。具体地说，在F1代（染色体加倍前），E-M和P-M引物分别导致46.3%和36.2%的小麦亲本带丢失和74.5%和68.4%的黑麦亲本带丢失。染色体加倍后的序列变异事件由连续的修饰组成，与染色体加倍前的变异速率相比，这些修饰以非常小的速率发生。然而，染色体加倍后的前5代中涉及黑麦亲本基因组的序列变异率比任何后续世代都高得多。令人惊讶的是，黑麦染色体加倍后发生的基因组变异的最高速率是在C-3或更晚，但不是在C-1。这些数据表明，细胞质和父母的基因组之间的关系的程度是决定的方向，数量，时间和基因组序列变异发生在属间异源多倍体化的速度的关键因素。
The timing and rate of genomic variation induced by allopolyploidization in the intergeneric wheat-rye (Triticum spp. - Secale cereale L.) hybrid triticale (x Triticosecale Wittmack) was studied using amplified fragment length polymorphism (AFLP) analyses with 2 sets of primers, EcoRI-MseI (E-M) and PstI-MseI (P-M), which primarily amplify repetitive and low-copy sequences, respectively. The results showed that allopolyploidization induced genome sequence variation in triticale and that a great degree of the genome variation occurred immediately following wide hybridization. Specifically, about 46.3% and 36.2% of the wheat parental band loss and 74.5% and 68.4% of the rye parental band loss occurred in the F-1 hybrids (before chromosome doubling) for E-M and P-M primers, respectively. The sequence variation events that followed chromosome doubling consisted of continuous modifications that occurred at a very small rate compared with the rate of variation before chromosome doubling. However, the rate of sequence variation involving the rye parental genome was much higher in the first 5 generations following chromosome doubling than in any subsequent generation. Surprisingly, the highest rate of rye genomic variation occurring after chromosome doubling was in C-3 or later, but not in C-1. The data suggested that the cytoplasm and the degree of the relationship between the parental genomes were the key factors in determining the direction, amount, timing, and rate of genomic sequence variation occurring during intergeneric allopolyploidization.