Functional expression of Δ12 fatty acid desaturase modulates thermoregulatory behaviour in Drosophila
Functional expression of Δ12 fatty acid desaturase modulates thermoregulatory behaviour in Drosophila
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DOI：
10.1038/s41598-020-68601-2
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发表时间：
2020-07
影响因子：
4.6
通讯作者：
Takuto Suito;K. Nagao;K. Takeuchi;N. Juni;Y. Hara;M. Umeda
中科院分区：
文献类型：
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作者：
Takuto Suito;K. Nagao;K. Takeuchi;N. Juni;Y. Hara;M. Umeda
多不饱和脂肪酸（PUFA）在多种动物和植物适应寒冷环境中起着至关重要的作用。然而，PUFAs影响体温调节行为的机制仍然难以捉摸。因此，我们研究了PUFAs在果蝇体温调节行为中的作用。为此，我们产生了表达秀丽隐杆线虫Δ12脂肪酸去饱和酶（FAT-2）的转基因果蝇，其将单不饱和脂肪酸转化为PUFA，例如亚油酸[C18：2（n-6）]和亚麻酸[C18：3（n-3）]。利用GAL 4/UAS表达系统对FAT-2进行神经元特异性表达，可提高中枢神经系统（CNS）中含C18：2（n-6）磷脂的含量，并显著降低三龄幼虫的嗜温性。在对表达温度感受器的神经元的遗传筛选和钙成像分析中，我们证明了在表达TRPA 1的神经元中FAT-2的异位表达通过调节神经元活动导致偏好温度的降低。我们的结论是，在一个子集的神经元的功能表达的FAT-2改变的温度调节行为的D。这可能是通过调节神经元细胞膜中含有PUFA的磷脂的量来实现的。
Polyunsaturated fatty acids (PUFAs) play crucial roles in adaptation to cold environments in a wide variety of animals and plants. However, the mechanisms by which PUFAs affect thermoregulatory behaviour remain elusive. Thus, we investigated the roles of PUFAs in thermoregulatory behaviour ofDrosophila melanogaster. To this end, we generated transgenic flies expressingCaenorhabditis elegansΔ12 fatty acid desaturase (FAT-2), which converts mono-unsaturated fatty acids to PUFAs such as linoleic acid [C18:2 (n-6)] and linolenic acid [C18:3 (n-3)]. Neuron-specific expression of FAT-2 using the GAL4/UAS expression system led to increased contents of C18:2 (n-6)-containing phospholipids in central nerve system (CNS) and caused significant decreases in preferred temperature of third instar larvae. In genetic screening and calcium imaging analyses of thermoreceptor-expressing neurons, we demonstrated that ectopic expression of FAT-2 in TRPA1-expressing neurons led to decreases in preferred temperature by modulating neuronal activity. We conclude that functional expression of FAT-2 in a subset of neurons changes the thermoregulatory behaviour ofD. melanogaster, likely by modulating quantities of PUFA-containing phospholipids in neuronal cell membranes.