Distribution and characteristics of slow-cycling cells in rat vocal folds
Distribution and characteristics of slow-cycling cells in rat vocal folds
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DOI：
10.1002/lary.25558
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发表时间：
2016-04-01
期刊：
影响因子：
2.6
通讯作者：
Hirano, Shigeru
中科院分区：
文献类型：
--
作者：
Kawai, Yoshitaka;Kishimoto, Yo;Hirano, Shigeru
干细胞在成人器官中增殖速度缓慢，因此在某些器官中表现出多能性的慢循环细胞被认为是组织特异性干细胞。慢循环细胞在声带（VF）尚未得到很好的记录。在这里，我们试图澄清大鼠VFs中慢循环细胞的分布和特征。方法采用双标记法检测慢循环细胞的分布。我们给Sprague-Dawley大鼠注射外源性增殖标志物5-溴-2′-脱氧尿苷（BrdU）。经过一段时间后，用BrdU抗体和第二种内源性增殖标志物Ki-67对VFs进行免疫染色。BrdU (+) Ki-67（+）细胞作为慢循环细胞，按VF区计数。为了揭示慢循环细胞的特征，我们对其免疫表型进行了组织学研究，并对其在损伤VFs中的动力学进行了评估。结果慢循环细胞以基底上皮细胞居多。与其他上皮细胞相比，上皮慢循环细胞中E-cadherin和CK5的阳性比例较低，而vimentin和CD31的阳性比例较高。S100A4在固有层和黄斑慢循环细胞中表达较低。在慢循环细胞中未检测到FGFR1、HAS1、HAS2和HAS3。在损伤的VFs中观察到慢循环细胞的时间依赖性减少。结论慢循环细胞大多存在于上皮内，在相对未分化的状态下表现出多种表型，可能参与损伤VFs的组织修复。证据水平喉镜，26 (6):564 - 571,2016
Objectives/HypothesisStem cells are known to proliferate at a slow rate in adult organs, and thus slow-cycling cells exhibiting pluripotency are considered tissue-specific stem cells in some organs. Slow-cycling cells in the vocal fold (VF) have not been well documented. Here we sought to clarify the distributions and characteristics of slow-cycling cells in rat VFs.MethodsWe applied double-labeling technique to detect the distribution of slow-cycling cells. We injected the exogenous proliferation marker 5-bromo-2'-deoxyuridine (BrdU) into Sprague-Dawley rats. After a chasing period, VFs were immunostained with antibodies to BrdU and the second endogenous proliferation marker, Ki-67. BrdU (+) Ki-67(+) cells were regarded as slow-cycling cells and counted by VF regions. To reveal slow-cycling cells' characteristic, their immunophenotypes were histologically investigated and their kinetics in injured VFs were evaluated.ResultsMost slow-cycling cells were detected in the basal layer of the epithelium. Slow-cycling cells in the epithelium displayed a low positive ratio of E-cadherin and CK5 and a high positive ratio of vimentin and CD31 as compared with the other epithelial cells. The expression of S100A4 was low in slow-cycling cells of the lamina propria and the macula flava. FGFR1, HAS1, HAS2, and HAS3 were not detected in the slow-cycling cells. A time-dependent reduction of slow-cycling cells was observed in injured VFs.ConclusionMost slow-cycling cells resided in the epithelium, exhibiting various phenotypes in a relatively undifferentiated condition, and they are suspected to contribute to the tissue repair of the injured VFs.Level of EvidenceN/A. Laryngoscope, 126:E164-E170, 2016