SUPEROXIDE-DEPENDENT FORMATION OF HYDROXYL RADICALS IN THE PRESENCE OF IRON SALTS - DETECTION OF FREE IRON IN BIOLOGICAL-SYSTEMS BY USING BLEOMYCIN-DEPENDENT DEGRADATION OF DNA
SUPEROXIDE-DEPENDENT FORMATION OF HYDROXYL RADICALS IN THE PRESENCE OF IRON SALTS - DETECTION OF FREE IRON IN BIOLOGICAL-SYSTEMS BY USING BLEOMYCIN-DEPENDENT DEGRADATION OF DNA
复制标题
DOI：
10.1042/bj1990263
复制
发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
HALLIWELL, B
中科院分区：
文献类型：
--
作者：
GUTTERIDGE, JMC;ROWLEY, DA;HALLIWELL, B
博莱霉素在Fe（II）存在下降解[小牛胸腺] DNA，形成硫代巴比妥酸反应产物。这已成为生物体液中游离铁的特定测定方法的基础。人滑液，人CSF和大鼠胸膜渗出液中含有微摩尔浓度的游离铁，这将足以使体内产生的超氧化物和H2O2的羟基自由基的形成。该测定方法不能检测与转运蛋白或酶结合的Fe。
Bleomycin in the presence of Fe(II) degrades [calf thymus] DNA to form a thiobarbituric acid-reactive product. This has been made the basis of a specific assay method for free Fe in biological fluids. Human synovial fluid, human CSF and rat pleural-exudate fluid contained micromolar concentrations of free Fe, which would be sufficient to allow formation of the hydroxyl radical from superoxide and H2O2 generated in vivo. This assay method does not detect Fe bound to transport proteins or to enzymes.