Thymic nurse cells exhibit epithelial progenitor phenotype and create unique extra-cytoplasmic membrane space for thymocyte selection
Thymic nurse cells exhibit epithelial progenitor phenotype and create unique extra-cytoplasmic membrane space for thymocyte selection
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DOI：
10.1016/j.cellimm.2009.11.004
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发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Guyden, Jerry C.
中科院分区：
文献类型：
--
作者：
Hendrix, Tonya M.;Chilukuri, Rajendra V. E.;Guyden, Jerry C.
胸腺哺育细胞(TnCs)是胸腺皮质中的上皮细胞，在特化的细胞质空泡中含有多达50个胸腺细胞。自1980年被发现以来，这种细胞间相互作用的功能一直引起争议。此外，对于TNC复合体中胸腺细胞的凋亡是负选择的结果的观点，也存在一些怀疑，这一过程被认为只发生在延髓内。在这份报告中，我们有微观证据表明，我们定义了一个独特的膜性环境，其中脂筏聚集在表达石油捕获的胸腺细胞和表达在TNCs上的II类MHC分子的α-βTCR周围。此外，胸腺切片的免疫组织学检查显示，位于皮质-髓质交界处的TNCs表达细胞角蛋白5和8(K5和K8)以及转录因子Trp-63，其表型在其他地方定义为胸腺上皮祖细胞亚群。我们的结果表明，跨国公司提供的微环境在胸腺细胞的选择中起着重要的作用，同时也可能参与胸腺上皮的维持。(C)2009 Elsevier Inc.保留所有权利
Thymic nurse cells (TNCs) are epithelial cells in the thymic cortex that contain as many as 50 thymocytes within specialized Cytoplasmic vacuoles. The function of this cell-in-cell interaction has Created controversy since their discovery in 1980. Further, some skepticism exists about the idea that apoptotic thymocytes within the TNC complex result from negative selection, a process believed to occur exclusively within the medulla. In this report, we have microscopic evidence that defines a unique membranous environment wherein lipid raft aggregates around the alpha beta TCR expressed oil captured thymocytes and class II MHC molecules expressed on TNCs. Further, immunohistological examination of thymic sections show TNCs located within the cortico-medullary junction to express cytokeratins five and eight (K5 and K8), and the transcription factor Trp-63, the phenotype defined elsewhere as the thymic epithelial progenitor subset. Our results suggest that the microenvironment provided by TNCs plays an important role in thymocyte selection as well as the potential for TNCs to be involved in the maintenance of thymic epithelia. (C) 2009 Elsevier Inc. All rights reserved