Measurement of optimal flow rate in gradient elution liquid chromatography
Measurement of optimal flow rate in gradient elution liquid chromatography
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DOI：
10.1016/j.chroma.2021.462645
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发表时间：
2021-10-31
影响因子：
4.1
通讯作者：
Blumberg, Leonid M.
中科院分区：
文献类型：
--
作者：
Foster, Samuel W.;Wright, Nicholas;Blumberg, Leonid M.
在梯度LC中的方法发展依赖于溶剂时间程序和流动相流速的选择。用于梯度分离的最佳流速不能通过给定分析物的最佳等规值来固有地预测，而应独立确定，以确保梯度分析的最高分离性能。最佳流速(F-opt)在此定义为使预定峰对的分离度(Delta值(S))或整个LC分析的分离量(S(C))最大化的溶剂体积流速(F)。讨论了梯度洗脱分析中F opt测量的理论背景和实验技术，并进行了实验验证。F(Op)t的测量技术是基于F的可平移变化，其中乘积Ft(G)(t(G)是梯度时间)对于F的所有值都是相同的。F-opt被发现为F对应于Delta(S)或S(C)的最大值。(C)2021年爱思唯尔B.V.保留所有权利。
Method development in gradient LC relies upon the selection of a solvent time program and a mobile phase flow rate. The flow rate, optimal for gradient separation cannot be inherently predicted by the isocratic value optimal for a given analyte, and rather should be identified independently to ensure the highest separation performance of gradient analysis. The optimal flow rate (F-opt) is defined herein as the solvent volumetric flow rate (F) maximizing the separation (Delta(s)) of a predetermined peak-pair or the separation capacity (s(c)) of the entire LC analysis. The theoretical background and the experimental technique of measurement of F opt in gradient elution analysis were considered and experimentally demonstrated. The technique of measuring F(op)t is based on translatable changes of F where the product Ft(G) (t(G) is the gradient time) was the same for all values of F. The F-opt was found as F corresponding to the maximum in Delta(s) or in s(c). (C) 2021 Elsevier B.V. All rights reserved.