FOURIER-TRANSFORM HOLOGRAPHIC MICROSCOPE
FOURIER-TRANSFORM HOLOGRAPHIC MICROSCOPE
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DOI：
10.1364/ao.31.004973
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发表时间：
1992-08-20
期刊：
影响因子：
1.9
通讯作者：
RHODES, CK
中科院分区：
文献类型：
--
作者：
HADDAD, WS;CULLEN, D;RHODES, CK
我们描述了一种空间分辨率接近衍射极限的全息显微镜。该仪器使用一小滴甘油作为透镜，产生傅里叶变换全息术所需的球面发散参考照明。通过对暗场照明刀口成像得到的点扩展函数的测量表明，在波长为514.5 nm的情况下，横向分辨率为1.4µm。通过光学切片的数值等效，从全息图中获得纵向分辨率。我们描述了重建的方法，并用选定的生物标本演示了显微镜的能力。该仪器提供了两种独特的功能：(1)它可以在单个光脉冲中收集三维信息，避免标本损坏和漂白；(2)它可以从一系列光脉冲中记录三维运动图像。概念设计适用于广泛的波长范围，我们讨论了对x射线区域的扩展。
We describe a holographic microscope with a spatial resolution approaching the diffraction limit. The instrument uses a tiny drop of glycerol as a lens to create the spherically diverging reference illumination necessary for Fourier-transform holography. Measurement of the point-spread function, which is obtained by imaging a knife edge in dark-field illumination, indicates a transverse resolution of 1.4-mu-m with wavelength lambda = 514.5 nm. Longitudinal resolution is obtained from the holograms by the numerical equivalent of optical sectioning. We describe the method of reconstruction and demonstrate the microscope's capability with selected biological specimens. The instrument offers two unique capabilities: (1) it can collect three-dimensional information in a single pulse of light, avoiding specimen damage and bleaching; and (2) it can record three-dimensional motion pictures from a series of light pulses. The conceptual design is applicable to a broad range of wavelengths and we discuss extension to the x-ray regime.