Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells
Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells
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DOI：
10.1016/j.molimm.2017.07.009
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发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Kitazawa, Haruki
中科院分区：
文献类型：
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作者：
Kanmani, Paulraj;Albarracin, Leonardo;Kitazawa, Haruki
先前的研究表明，德氏乳杆菌OLL 1073 R-1（LDR-1）产生的胞外多糖（EPS）可提高抗病毒免疫力，尤其是在全身和呼吸系统。然而，以前没有研究这些EPS是否能够有益地调节肠道抗病毒免疫。此外，LDR-1与宿主的相互作用主要是用免疫细胞进行评价的，而其与肠上皮细胞（IEC）的相互作用以前没有得到解决。在这项工作中，我们研究了来自LDR-1的EPS调节猪IEC（PIE细胞）对Toll样受体（TLR）-3激动剂poly（I：C）刺激的反应的能力，以及TLR 2、TLR 4和TLR负调节剂在免疫调节作用中的作用。我们发现，在猪IEC中由TLR 3激活触发的先天免疫应答受到来自LDR-1的EPS的差异调节。EPS处理诱导PIE细胞中干扰素（IFN）-α和IFN-13在用poly（I：C）刺激后的表达增加，以及抗病毒因子MxA和RNase L的表达增加。这些作用与EPS处理的PIE细胞中A20表达减少有关。来自LDR-1的EPS也能够降低IL-6和促炎趋化因子的表达。虽然还需要进一步的体内研究，但我们的研究结果表明，这些EPS或用LDR-1发酵的酸奶有可能改善肠道先天性抗病毒反应并保护免受肠道病毒的侵害。
Previous studies demonstrated that the extracellular polysaccharides (EPSs) produced by Lactobacillus delbrueckii OLL1073R-1 (LDR-1) improve antiviral immunity, especially in the systemic and respiratory compartments. However, it was not studied before whether those EPSs are able to beneficially modulate intestinal antiviral immunity. In addition, LDR-1-host interaction has been evaluated mainly with immune cells while its interaction with intestinal epithelial cells (IECs) was not addressed before. In this work, we investigated the capacity of EPSs from LDR-1 to modulate the response of porcine IECs (PIE cells) to the stimulation with the Toll-like receptor (TLR)-3 agonist poly(I:C) and the role of TLR2, TLR4, and TLR negative regulators in the immunoregulatory effect. We showed that innate immune response triggered by TLR3 activation in porcine IECs was differentially modulated by EPS from LDR-1. EPSs treatment induced an increment in the expression of interferon (IFN)-alpha and IFN-I3 in PIE cells after the stimulation with poly(I:C) as well as the expression of the antiviral factors MxA and RNase L. Those effects were related to the reduced expression of A20 in EPS-treated PIE cells. EPS from LDR-1 was also able to reduce the expression of IL-6 and proinflammatory chemokines. Although further in vivo studies are needed, our results suggest that these EPSs or a yogurt fermented with LDR-1 have potential to improve intestinal innate antiviral response and protect against intestinal viruses.